Efficiency of RAPD, ISSR, iPBS, SCoT and phytochemical markers in the genetic relationship study of five native and economical important bamboos of North-East India.

Genetic analysis of Penthorum chinense Pursh by improved RAPD and ISSR in China.

Thunbergia coccinea Wall. ex D. Don being a rare, ornamental and medicinal plant of India, is needed to propagate for conserving the germplasm and analyzing its phytochemical compounds in the future. A reliable protocol for direct in vitro propagation using nodal shoot meristem of T. coccinea as explant was standardized. The highest number of shoots per explant (22.17 ± 0.54) with maximum shoot length (2.36 ± 0.28) in cm was obtained in Murashige and Skoog (MS) medium supplemented with 9.70 µM of 6-furfurylaminopurine (Kinetin) and 0.053 µM of α-naphthaleneacetic acid (NAA) combination, among all the different plant growth regulators (PGR's) and concentrations tested. The aforesaid PGR's combination was optimum for axillary shoot bud induction and multiplication in T. coccinea. The best rooting was observed on the half-strength MS medium fortified with 2.68 µM NAA with the highest number of roots per shoot (3.75 ± 0.12) and maximum length (5.22 ± 0.32) in cm. All the in vitro raised plantlets were acclimatized in sterile sand and soil mixture (1:1) with a survival rate of 70% on earthen pots under greenhouse conditions. PCR-based RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat) molecular markers were employed to determine the genetic homogeneity amongst the plantlets. Twelve (12) RAPD and nine (9) ISSR primers developed a total of 104 and 91 scorable bands, respectively. The band profiles of micropropagated plantlets were monomorphic to the mother, donor in vivo plant, and similarity values varied from 0.9542-1.000. The dendrogram generated through UPGMA (unweighted pair group method with arithmetic mean) showed 99% similarities amongst all tested plants confirming the genetic uniformity of in vitro raised plants. 

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Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Galdieria is a photosynthetic unicellular protist, inhabiting thermoacidic environments around the world. The synchronicity of these thermoacidophilic algae with their extreme habitats makes them unable to thrive in different ecological conditions. The genetic structure of Galdieria populations has not yet been studied. In this report, the level of genetic diversity and structure of five Galdieria populations from Iceland were assessed through both random amplified polymorphic DNA (RAPD) markers, and a partial calmodulin gene fragment (previously used to assess the population structure of these extremophilic algae). The level of population differentiation from both the RAPD and CaM markers was estimated using PLP, percentage of polymorphic loci; H, the expected heterozygosity; I, Shannon’s information index, Ks, Kst. The migration ability of the Galdieria populations was suggested by the high level of genetic variations scored within each Galdieria population and by the small number of polymorphisms detected between the different Icelandic populations. The low genetic diversity between the two species was highlighted by RAPD and calmodulin markers, suggesting for the first time an interspecific genetic flow between species as strategy to evolve in stressful environments.

Genetic structure of Galdieria populations from Iceland

Polygonatum sibiricum is widely consumed as a traditional Chinese herb and edible plant in China Despite its nutritional and medical values, research on Polygonatum Mill has been scarce, particularly as far as its genetic diversity is concerned In this study, fourteen expressed sequence tag-derived simple sequence repeat (EST-SSR) and seven sequence-related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversity in fifty Polygonatum Mill accessions The EST-SSRs and SRAPs produced 173 (9058%) and 113 (9339%) polymorphic bands, respectively Unweighted Pair-Group Method Analysis (UPGMA) based on the combined data matrices of EST-SSRs and SRAPs divided the fifty Polygonatum Mill accessions into fourteen groups In addition, accessions of P cyrtonema Hua obtained from Anhui and Zhejiang provinces were clustered according to their geographic origin Furthermore, some accessions were gathered together based on species, such as P kingianum Coll et Hemsl, P punctatum Royle ex Kunth, P odoratum (Mill) Druce, and P sibiricum Red, and bootstrap analysis for clustering fully supported the grouping of the accessions The Analysis of Molecular Variance (AMOVA) results revealed higher variation within populations (95%) rather than among populations (5%), indicating that Polygonatum Mill has a low genetic differentiation between populations, and Principal Coordinate Analysis (PCoA) greatly supported the results of cluster analysis and AMOVA analysis Finally, five markers which could produce abundant and stable bands were used to construct DNA fingerprinting database of Polygonatum Mill Our results demonstrated the utility of both EST-SSR and SRAP markers to successfully evaluate and identify Polygonatum Mill

Evaluation of genetic diversity and construction of DNA fingerprinting in Polygonatum Mill. based on EST-SSR and SRAP molecular markers

Determination of genetic diversity among Saccharina germplasm using ISSR and RAPD markers.

The invention discloses a caulerpa lentillifera cultivation device and a cultivation method thereof The cultivation device comprises a cultivation pond and a cultivation frame, wherein the cultivation frame comprises a frame body, a bottom net and a cover net, the bottom net is fixed to the bottom face of the frame body, and the cover net is fixed to the top face of the frame body; supporting rods are arranged at the two ends of the cultivation pond, two or more supporting cables are installed between the two supporting rods, and the cultivation frame is placed on the supporting cables The cultivation method includes the steps that caulerpa lentillifera female parents are laid on the bottom net in a scattered mode and covered with the cover net to be fixed, the cultivation frame is horizontally placed on the supporting cables in the cultivation pond, the illumination intensity is 4000lx-5000lx, the ratio of illumination L/darkness D is 12h/12h, the molar ratio of nitrogen to phosphorus to potassium in cultivation seawater is 3:1:1-6:1:1, the concentration of nitrogen in the cultivation seawater is 6192 mg/L, the concentration of phosphorus in the cultivation seawater is 228 mg/L, the concentration of potassium in the cultivation seawater is 287 mg/L, and water temperature is 25 DEG C-27 DEG C The cultivation method can improve the growth efficiency of caulerpa lentillifera and product quality

Caulerpa lentillifera cultivation device and cultivation method thereof

Zotera marina microsatellite markers, screening method thereof, and application thereof

The invention discloses a method for rapidly preparing a PCR template for kelp sporophore seedlings and an amplifying method of the PCR template. The method comprises the following steps: fetching thekelp sporophore seedlings, cutting leaves, and putting the leaves into a centrifuge tube; adding distilled water for soaking the leaves of the seedlings, and adequately grinding the leaves of the seedlings, carrying out centrifugation; and transferring supernate into a new centrifuge tube as a PCR temperature for amplification. According to the method, the steps are obviously simplified on the basis that genomic DNA is conventionally extracted for PCR detection, various reagents and kits do not need to be used, the genomic DNA does not need to be extracted, the kelp sporophore seedlings are directly ground and centrifuged, supernate is directly extracted for PCR amplification, so that the method is simple and convenient, the result is definite, a large number of manpower, material resources and financial resources are saved, and the pollution and injury which are caused to the environment and experimenters are greatly reduced.

Preparation method of PCR template for kelp sporophore seedlings and amplifying method

The invention belongs to the field of molecular genetics of seaweed molecules, and particularly relates to a kelp gametophyte sex determination method. The kelp gametophyte sex determination method ischaracterized in that a specific molecular marker is respectively provided for a female gametophyte and a male gametophyte of a kelp, and the specific molecular markers are respectively named as SJFand SJM; nucleotide sequences of the specific molecular marker are respectively SEQ ID NO: 5 and SEQ ID NO: 6. The SEQ ID NO: 5 is obtained by amplifying the sequence SEQ ID NO: 3 of a primer Sf; theSEQ ID NO: 6 is obtained by amplifying the sequence SEQ ID NO: 4 of a primer Sm. The gametophyte sex determination method is completed by detecting the specific fragment, and comprises the steps of extraction of a genome DNA of a kelp gametophyte to be detected, PCR amplification and agarose gel electrophoresis detection of an amplification product. In conclusion, the method can definitely determine that whether the kelp gametophyte is pure female, pure male or hybrid male and female, and overcomes the shortcomings in the prior art.

Peng Jie

Papers

Determination of genetic diversity among Saccharina germplasm using ISSR and RAPD markers.

Caulerpa lentillifera cultivation device and cultivation method thereof

Zotera marina microsatellite markers, screening method thereof, and application thereof

Preparation method of PCR template for kelp sporophore seedlings and amplifying method

Kelp gametophyte sex determination method