Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves.

Proteins are now widely produced in diverse microbial cell factories. The Escherichia coli is still the dominant host for recombinant protein production but, as a bacterial cell, it also has its issues: the aggregation of foreign proteins into insoluble inclusion bodies is perhaps the main limiting factor of the E. coli expression system. Conversely, E. coli benefits of cost, ease of use and scale make it essential to design new approaches directed for improved recombinant protein production in this host cell.With the aid of genetic and protein engineering novel tailored-made strategies can be designed to suit user or process requirements. Gene fusion technology has been widely used for the improvement of soluble protein production and/or purification in E. coli, and for increasing peptide’s immunogenicity as well. New fusion partners are constantly emerging and complementing the traditional solutions, as for instance, the Fh8 fusion tag that has been recently studied and ranked among the best solubility enhancer partners. In this review, we provide an overview of current strategies to improve recombinant protein production in E. coli, including the key factors for successful protein production, highlighting soluble protein production, and a comprehensive summary of the latest available and traditionally-used gene fusion technologies. A special emphasis is given to the recently discovered Fh8 fusion system that can be used for soluble protein production, purification and immunogenicity in E. coli. The number of existing fusion tags will probably increase in the next few years, and efforts should be taken to better understand how fusion tags act in E. coli. This knowledge will undoubtedly drive the development of new tailored-made tools for protein production in this bacterial system.

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Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system

Diarrhoeal diseases, almost all of which are caused by food-borne or waterborne microbial pathogens, are leading causes of illness and death in less developed countries, killing an estimated 1.9 million people annually at the global level. Even in developed countries, it is estimated that up to one third of the population are affected by microbiological food-borne diseases each year. The majority of the pathogens causing this significant disease burden are now considered to be zoonotic. The occurrence of some of these zoonotic pathogens seems to have increased significantly over recent years. The factors involved in such increases have not been well studied, but they are generally agreed to include changes in animal production systems and in the food production chain. Both types of changes can cause corresponding changes in patterns of exposure to the pathogens and the susceptibility pattern of the human population. This paper will not attempt a more in-depth analysis of such factors. The authors briefly describe five of the most important emerging food-borne zoonotic pathogens: Salmonella spp., Campylobacter spp., enterohaemorrhagic Escherichia coli, Toxoplasma gondii and Cryptosporidium parvum. The paper does not include a full description of all important emerging food-borne pathogens but instead provides a description of the present situation, as regards these globally more important pathogens. In addition, the authors describe each pathogen according to the new framework of a Food and Agriculture Organization (FAO)/World Health Organization (WHO) microbiological risk assessment, which consists of hazard identification and characterisation, exposure assessment and risk characterisation. Moreover, the authors provide a brief account of attempts at risk mitigation, as well as suggestions for risk management for some of these pathogens, based on thorough international FAO/WHO risk assessments. The authors emphasise the importance of science-based programmes for the continued reduction of pathogens at relevant points of the 'farm-to-fork' food production chain, as this is the only sustainable basis for further reducing risks to human health in the area of preventable food-borne diseases.

Emerging food-borne zoonoses.

Cryptosporidium molnari n. sp. (Apicomplexa: Cryptosporidiidae) infecting two marine fish species, Sparus aurata L. and Dicentrarchus labrax L.

https://archive-ouverte.unige.ch/unige:78032/ATTACHMENT01

Molecular and functional aspects of parasite invasion

Cryptosporidium parvum 検出技術の開発

微細加工技術を利用して、試料中の大量の細胞を、ダメージを与えることなく高効率で１細胞レベルで分離・捕捉するマイクロ流路デバイス（microfluidicdevice）を提供するものである。 試料供給口と試料排出口を連通するマイクロ流路形成用の溝が設けられたプラスチック製平板からなる上部基板と、前記上部基板と協働してマイクロ流路を形成するとともに、マイクロメッシュ用開口窓が設けられたプラスチック製上部平板と、細胞捕捉用の複数の微細貫通孔を有するプラスチック製のマイクロメッシュを保持した保持平板と、吸引用開口窓が設けられたプラスチック製下部平板と、吸引流路形成用の溝が設けられたプラスチック製平板からなる下部基板とを順次備え、前記マイクロメッシュの細胞捕捉用の微細貫通孔の形状が反すり鉢状又は円筒状であり、前記上部平板を前記上部基板に比較して軟質のプラスチックで構成する。

朋之田口

Papers

Cryptosporidium parvum 検出技術の開発

Microfluidic device for single-cell trapping

朋之 田口

Papers

Cryptosporidium parvum 検出技術の開発

Microfluidic device for single-cell trapping

朋之田口