P
Peter Brne
Researcher at BIA Separations (Slovenia)
Publications - 8
Citations - 350
Peter Brne is an academic researcher from BIA Separations (Slovenia). The author has contributed to research in topics: Monolithic HPLC column & Ion chromatography. The author has an hindex of 8, co-authored 8 publications receiving 330 citations.
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Convective interaction media short monolithic columns: enabling chromatographic supports for the separation and purification of large biomolecules.
TL;DR: Convective Interaction Media short monolithic columns (SMC) that are based on methacrylate monoliths exhibit some interesting features that make them attractive for these tasks, and applications of SMC for the separation and purification of large biomolecules are presented.
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Methacrylate‐based short monolithic columns: Enabling tools for rapid and efficient analyses of biomolecules and nanoparticles
Miloš Barut,Aleš Podgornik,Lidija Urbas,Boštjan Gabor,Peter Brne,Jana Vidič,Sašo Plevčak,Aleš Štrancar +7 more
TL;DR: The efficiency of the monolithic column is demonstrated--where the mass transfer between the stationary and mobile phase is greatly enhanced--for the in-process and final control of the new therapeutics.
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Simple method for determining the amount of ion-exchange groups on chromatographic supports
TL;DR: It was concluded that for a given monolith the protein capacity can be derived from the data obtained by the new method, which was shown to be suitable for determining the amount of ionic groups on both anion and cation monolithic columns.
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Characterization of ion exchange stationary phases via pH transition profiles.
TL;DR: The duration of pH transient was shown to be linearly proportional to the total ionic capacity and was used to estimate protein dynamic binding capacity of the resin and to distinguish between cation and anion or weak and strong ion exchange chromatographic supports.
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Depletion of high-abundance proteins from human plasma using a combination of an affinity and pseudo-affinity column.
Lidija Urbas,Peter Brne,Boštjan Gabor,Miloš Barut,Matija Strlič,Matija Strlič,Tatjana Čerk Petrič,Aleš Štrancar +7 more
TL;DR: The optimization of conditions for selective depletion of HSA and IgG using affinity and pseudo-affinity chromatography and two-dimensional gel electrophoresis is described.