P
Phillip Williams
Researcher at University of Wisconsin-Madison
Publications - 17
Citations - 6003
Phillip Williams is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Gene expression & Expression vector. The author has an hindex of 15, co-authored 17 publications receiving 5674 citations.
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Journal ArticleDOI
Direct gene transfer into mouse muscle in vivo.
Jon A. Wolff,Robert W. Malone,Phillip Williams,Wang Chong,Gyula Acsadi,Agnes Jani,Philip L. Felgner +6 more
TL;DR: RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and beta-galactosidase were separately injected into mouse skeletal muscle in vivo and expression was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions.
Journal ArticleDOI
Direct Gene Transfer into Nonhuman Primate Myofibers In Vivo
Shoushu Jiao,Phillip Williams,Randi K. Berg,Bradley A. Hodgeman,Lijian Liu,Gabriela M. Repetto,Jon A. Wolff +6 more
TL;DR: It is demonstrated that nonhuman primate muscle also has this ability to express injected plasmid genes injected intramuscularly, and the luciferase expression was considerably less than that in rodents.
Journal ArticleDOI
Plasmid DNA entry into postmitotic nuclei of primary rat myotubes
TL;DR: Understanding of plasmid DNA nuclear transport provides a basis for increasing the efficiency of gene transfer.
Journal ArticleDOI
Establishment of hamster blastocyst-derived embryonic stem (ES) cells
TL;DR: The establishment of four ES cell lines from the Syrian "golden" hamster (Mesocricetus auratus) is described, which should be useful for developmental and transgenic studies.
Journal ArticleDOI
Expression of naked plasmids by cultured myotubes and entry of plasmids into T tubules and caveolae of mammalian skeletal muscle.
Jon A. Wolff,Martin E. Dowty,Shoushu Jiao,Gabriella Repetto,Randi K. Berg,James J. Ludtke,Phillip Williams,David B. Slautterback +7 more
TL;DR: The results indicate that it is highly unlikely that the plasmid DNA enters the myofiber simply by the needle grossly disrupting the sarcolemma, and transient membrane disruptions do not appear to be responsible for the uptake of DNA.