Showing papers by "Pramod Kumar Tiwari published in 2007"
TL;DR: The involvement of the HSP60 and HSP70 proteins in the development, growth and differentiation of both somatic and germ line tissues is indicated and the enhanced co-expression of H SP70 and H SP60 upon heat shock in various larval and adult cell types suggests the possible role of HSP 60 in thermoprotection.
Abstract: The expression pattern of two major chaperones, the heat shock proteins (HSPs) HSP60 and HSP70 was studied in vitro in tissues of the housefly Musca domestica during larval and adult stages of development to identify their immunological relatives and understand their functional significance in normal cellular activities and during thermal stress. Fluorographs of labeled polypeptides and western blots demonstrated that both HSPs are expressed constitutively and heat-induced in all the larval and adult cell types examined. The pattern of whole tissue immunocytochemical staining using anti-HSP60 and anti-HSP70 antibodies corresponded well with the observations from western blots or fluorographs. In developing oocytes, both constitutive and heat inducible expression of HSP60 were regulated in an oocyte stage-specific manner. In unstressed ovaries the expression of these proteins was less pronounced in early stage oocytes (1st - 8th) than at later stages (9th and onward). The heat shock, however, induced both HSP70 and HSP60 to a significantly high level in early stage oocytes (1st-8th) as compared to their respective controls. Our findings indicate the involvement of the HSP60 and HSP70 proteins in the development, growth and differentiation of both somatic and germ line tissues. Furthermore, the enhanced co-expression of HSP70 and HSP60 upon heat shock in various larval and adult cell types suggests the possible role of HSP60 in thermoprotection.
16 citations
TL;DR: A strong association ofLDH3 with sputum three positive or severe cases of mycobacterial infection is suggested, indicating a likely possibility of using LDH3 as a supporting diagnostic marker in, at least, cases of chronic tuberculosis.
13 citations
Journal Article•
TL;DR: Interestingly, blood group “O” and “AB” were found significantly associated with “whorl”and “loop” fingerprint pattern types in each cohort, and blood group’s dominant allele for hand clasping and thumb extension traits was dominant while recessive allele for the same was dominant.
Abstract: The present study was carried out to understand the genetic relationship if any, among different Indian populations using classical dermatoglyphic markers in 250 post-graduate students. Finger print patterns were collected on a white paper using a non-spreading blue ink-pad. The other traits were recorded by direct observation. The ridges were counted and patterns were identified using glass hand-lens. Statistical analysis was done using student t-test, two-way anova (multivariate) and chi-square test of significance using ANALYSE-IT software. Phylogenetic analysis was carried out using PopGen 32 and MEGA 4 software. Significant variations were found for total ridge count between North vs. East cohort (p<0.001) and East vs. West cohort (p<.001). Interestingly, 55% of the participants from Northern cohort had dominant allele for hand clasping and thumb extension traits, while 55% participants of South cohort have recessive allele for the same. Interestingly, blood group “O” and “AB” were found significantly ((p= 0.07) associated with “whorl” and “loop” fingerprint pattern types in each cohort.
13 citations
TL;DR: The Sahariya tribe who live in various districts of Madhya Pradesh, central India are vulnerable to infections and other health hazards, and as a result, tuberculosis is prevalent in this tribe.
Abstract: I would like to draw your readers’ attention to the plight of the Sahariya tribe who live in various districts of Madhya Pradesh, central India. Sahariya means ‘inhabitants of the jungle’ (from the Persian word Sehr), and most of the year these people live in small villages called ‘sahrana’, dependent on the forest for produce and shelter (Figs1,2). However, for approximately 3 months of the year the tribe travels in search of work. At this time they are vulnerable to infections and other health hazards. As a result, tuberculosis (TB) is prevalent in this tribe.
4 citations