Capture-SELEX for aptamer selection: A short review.

Graphene oxide (GO) has attracted substantial interest in various fields of science and technology, owing to its remarkable mechanical, electrical and optical properties. It is prepared using the modified Hummers' method and employed as a precursor for the fabrication of graphene-based nanostructure materials. The facile synthesis of reduced graphene oxide and surface-modified GO using GO offers extensive opportunities in analytical chemistry. The surface modification of GO is realized via elemental doping and covalent and non-covalent bonding. It results in the functionalization of GO with different groups such as C–N, N N, N–H, and C–O–C. GO-based sensor technologies are extensively applied to identify numerous trace level analytes. These fluorescent, and electrochemical sensors are utilized primarily for detecting organic, inorganic, and biomolecules and for humidity sensing and bioimaging. The present review provides a holistic summary of not only the important literary aspects associated with GO but also the diverse practical applications of GO-based analytical strategies for the assaying of various target molecules.

Surface modifications and analytical applications of graphene oxide: A review

The problem of drug resistance of food borne pathogens is becoming more and more serious. Although traditional antimicrobial agents have good therapeutic effects on a variety of food borne pathogen...

Synergistic interactions of plant essential oils with antimicrobial agents: a new antimicrobial therapy.

Nanoemulsion-based edible coatings loaded with fennel essential oil/cinnamaldehyde: characterization, antimicrobial property and advantages in pork meat patties application

Inhibitory effect of cinnamaldehyde on Fusarium solani and its application in postharvest preservation of sweet potato.

The antifungal effects of cinnamaldehyde against Aspergillus niger and its application in bread preservation

We developed a fluorescent aptamer/graphene oxide (GO)–based biosensor to detect sulfamethazine (SMZ) residues in animal-derived foods. The SMZ-bound aptamers were identified and screened with an improved GO-SELEX technique using non-immobilizing ssDNA library. After seven rounds of selection, six SMZ aptamers were sequenced and analyzed for secondary structure, and their affinity and specificity were assessed by binding assays. The truncated aptamer (SMZ1S: 5′-CGTTAGACG-3′) with a unique stem-loop structure showed the highest affinity (Kd = 24.6 nM) to SMZ and was used to develop a GO-based fluorescent aptasensor. The binding mechanism between SMZ1S and SMZ was further analyzed by molecular docking. Under optimal conditions, the fluorescent aptasensor showed low detection limits (0.35 ng/mL) and a wide dynamic linear range (from 2 to 100 ng/mL). The aptasensor was also validated against real samples spiked with SMZ, which showed a fluorescence recovery from 93.9 to 108.8% and a coefficient of variation of < 12.7%. Taken together, these results suggest that this novel aptasensor can be used to sensitively, selectively, and accurately detect SMZ residues in foods.

Selection and truncation of aptamers for ultrasensitive detection of sulfamethazine using a fluorescent biosensor based on graphene oxide

Single-stranded DNA aptamers that specifically bind to sulfaquinoxaline (SQX) were selected by a graphene oxide–based SEL EX (GO-SELEX) technique assisted by a non-immobilizing TAMRA-labeled oligonucleotide library. After 8 rounds of selection against SQX, two aptamer candidates (i.e., SBA 1 and SBA 2) were obtained. Furthermore, the two full-length aptamers were truncated to obtain the aptamers (i.e. SBA 1-1, SBA 2-1, and SBA 2-2). These candidate aptamers were subjected to binding assays to evaluate their binding affinities and specificities to SQX. Our results show that the dissociation constants Kd of the aptamers ranged from 82.54 to 630.41 nM. Using truncated aptamer SBA 2-1 with the highest affinity as the recognition element, a GO-based fluorescent aptasensor was developed for SQX detection with a linear range from 0.05 to 50 ng mL−1 and a limit of detection of 0.11 ng mL−1 with excellent selectivity. Furthermore, the new aptasensor was used to detect SQX in milk samples. Our results showed that the aptasensor demonstrated recoveries ranging from 96.6 to 106.7%, suggesting that the proposed GO-based fluorescent aptasensor holds great potential as promising tool for sensitive detection of SQX in food safety inspection.

Selection and Application of DNA Aptamers Against Sulfaquinoxaline Assisted by Graphene Oxide–Based SELEX

A 2D/3D g-C3N4/ZnO heterojunction enhanced visible-light driven photocatalytic activity for sulfonamides degradation

Development of monoclonal antibody-based ultrasensitive enzyme-linked immunosorbent assay and fluorescence-linked immunosorbent assay for 1-aminohydantoin detection in aquatic animals

Qi Sun

Papers

The antifungal effects of cinnamaldehyde against Aspergillus niger and its application in bread preservation

Selection and truncation of aptamers for ultrasensitive detection of sulfamethazine using a fluorescent biosensor based on graphene oxide

Selection and Application of DNA Aptamers Against Sulfaquinoxaline Assisted by Graphene Oxide–Based SELEX

A 2D/3D g-C3N4/ZnO heterojunction enhanced visible-light driven photocatalytic activity for sulfonamides degradation

Development of monoclonal antibody-based ultrasensitive enzyme-linked immunosorbent assay and fluorescence-linked immunosorbent assay for 1-aminohydantoin detection in aquatic animals