The invention discloses an establishing method of a primary generation humanized mammary cancer transplantation tumor model based on Chinese population. The method comprises the following steps: (1) acquiring a human mammary cancer tissue sample; (2) performing inoculation for the first time; (3) culturing a mouse of the first inoculation; (4) performing inoculation for the second time; (5) culturing a mouse of the second inoculation; (6) performing inoculation for the third time; and (7) culturing a mouse of the third inoculation. The invention also discloses the primary generation humanized mammary cancer transplantation tumor model based on the Chinese population, which is obtained by the method. The primary generation humanized mammary cancer transplantation tumor model based on the Chinese population can be used for screening anti-mammary cancer medicaments and used as a test animal in a pharmacodynamics research. The primary generation humanized mammary cancer transplantation tumor model disclosed by the invention overcomes the problem that the existing transplantation tumor model in the Chinese population cannot truly reflect biological characteristics of the mammary cancer in a human body. Moreover, compared with the establishment of a foreign primary generation humanized mammary cancer related model, the primary generation humanized mammary cancer transplantation tumor model disclosed by the invention is used for realizing long-term and stable stimulation on the growth of primary generation humanized mammary cancer transplantation tumor and improving the method for establishing the model.

Establishing method of primary generation humanized mammary cancer transplantation tumor model based on Chinese population and model

The invention relates to the field of biology, and discloses a method for evaluating immunodeficiency degree of an immunodeficient mice model. The method comprises: through transplanting neoplastic hematologic disorder cells and solid tumor cells in different orders of magnitudes into an immunodeficient mice model, establishing a tumor mice model, detecting the proportion of tumor cells GPB in internal and external blood of the tumor mice model, the proportion of tumor cells GBM in bone marrow, and the proportion of tumor cells GSP in spleen, according to proportion parameter indexes, evaluating the immunodeficiency degree of the immunodeficient mice model. The method is advantaged in that the method can quantitatively evaluate defect level of the immunodeficient mice, the method relates to simplify and summarize a mathematic model and a calculation method which are obtained through relations between tumor transplanting efficiency and mice immunodeficiency degree, and through scores of immunodeficiency degree, defect difference degree among the immunodeficient mice can be compared. The method is simple and feasible, and is easy to operate, and the method has good application prospect.

Method for evaluating immunodeficiency degree of immunodeficient mice model

The invention discloses a method for building a triple negative breast cancer living tumor tissue mouse orthotopic transplantation model The method comprises the following steps of (1) subcutaneouslyembedding an estradiol sustained-release tablet in the neck of an immunodeficient mouse; (2) selecting triple negative breast cancer living tumor tissues of a clinical triple negative breast cancer patient through an operation, and then cutting the triple negative breast cancer living tumor tissues into inoculation tumor blocks with the volume being 4 to 15 mm ; (3) performing orthotopic transplantation on the inoculation tumor blocks into a mammary gland fat pad of the immunodeficient mouse; and (4) raising the immunodeficient mouse for 30 to 80 days, and building the triple negative breast cancer living tumor tissue mouse orthotopic transplantation model The method has the advantages that the estrogen level in a female mouse model body can be improved; and the growth requirement of the triple negative breast cancer tissues is met, so that the tumor nodule emergence time is early, the model building time is shortened, the tumor forming rate is improved by nearly 4 times, and the model building method is simple and feasible

Method for building triple negative breast cancer living tumor tissue mouse orthotopic transplantation model

The present disclosure provides methods of conducting and analyzing mouse clinical trials. In one embodiment, the method comprises the steps of receiving a dataset of tumor volumes measured in a mouse clinical trial, determining tumor growth curve of the treatment group and tumor growth curve of the control group; determining area under curve (AUC) of the treatment group and AUC of the control group; and evaluating efficacy of the drug based on an AUC ratio between the AUC of the treatment group and the AUC of the control group, wherein the mouse clinical trial comprises the steps of: obtaining a tumor sample derived from a patient; grafting the tumor sample to a treatment group comprising m mice and a control group comprising n mice, wherein m and n are integers; treating the treatment group with a drug; treating the control group with a vehicle; and measuring tumor volume of the treatment group and tumor volume of the control group at a plurality of days.

Methods of mouse clinical trial

The present invention relates to the technical field of medical evaluation and detection. The invention discloses a method to prepare a breast cancer immunodeficiency mouse model, which establishes a human breast cancer cell Nod-SCID mouse model firstly and successfully at home and abroad by regular injection of estrogen preparations to the immunodeficiency mice and transplant of the breast cancer cells after thereof. The breast cancer immunodeficiency mouse model constructed by the invention has short construction period, high tumor formation rate, low animal mortality, simple and easy construction method and low cost, and thus is an ideal breast cancer immunotherapy animal model.

Quanhai Liu

Papers

Mammary cancer immunodeficiency type animal model and method for preparing the same