http://xrm.phys.northwestern.edu/research/pdf_papers/2005/lerotic_jesrp_2005.pdf

Cluster analysis in soft X-ray spectromicroscopy : Finding the patterns in complex specimens

In scanning soft X‐ray microscopy a quantitative map of specimen absorptivity is obtained. Sharp changes in the transmission spectrum at absorption edges provide contrast and are the basis for elemental analysis by comparison of images taken at differing wavelengths. We have developed an instrument that uses monochromatized synchrotron radiation as its source. A collimated portion of this source is focused with a Fresnel zone plate to a submicron spot, across which the specimen is scanned under computer control. To illustrate the microscope’s capabilities, we have imaged whole cultured cells in a fixed but wet and unstained state, and measured the distribution of calcium in thin sections of human bone tissue.

R. Cave

Papers

Scanning soft X-ray microscopy a the national synchrotron light source