R. J. Mayer

TL;DR: In this paper, immunocytochemistry was performed on paraffin sections of tissues showing well-characterized inclusion bodies and conjugated ubiquitin was found as a component of the intermediate filament inclusion bodies characteristic of several major diseases.

TL;DR: Using an immunocytochemical method to localise antibodies to ubiquitin, filamentous inclusion bodies were seen in spinal anterior horn neurones in cases of motor neurone disease (MND) but not in any control cases, linking the protein ubiquitine with a chronic neurodegenerative disease.

TL;DR: Staining of normal human tissues reveals immunoreactivity of lens capsular epithelium, skeletal muscle, cardiac muscle, smooth muscle, and glial cells, as has been described by other workers.

TL;DR: The new technique of anti-ubiquitin immunocytochemistry has been used in a correlative quantitative neuropathological study of fifteen cases of diffuseLewy body disease, showing that the severity of dementia is related to cortical Lewy body density, whilst subcortical abnormalities make a much less significant contribution.

TL;DR: Quantitative studies show that the novel neuropathological technique of anti-ubiquitin immunocytochemistry is more than twice as sensitive as conventional haematoxylin and eosin stains in detecting cortical Lewy bodies.