In this study, antioxidant, cytotoxic, larvicidal, antimicrobial and anthelmintic effects and phenolic contents of ethanol, methanol and acetone extracts of leaf and tuber parts of Cyclamen alpinum were investigated. DPPH, ABTS, β-carotene assays were carried out in antioxidant activity and total phenolic and flavonoid contents were tested in determination assay. 9 phenolic contents were determined by HPLC. Artemia salina was used in the cytotoxic effect. Larvicidal effect was investigated against Culex pipiens. Disc diffusion method was used in antimicrobial effect. The tuber part was found to be more toxic than the leaf part in the anthelmintic activity assay. The highest value obtained from the antioxidant activity experiment was observed with value of 86.73 ± 0.16 (%) in DPPH assay. The lowest LC50 value in larvicidal effect was determined 0.151 mg/mL after 72 hours. Consequently, there is need for further studies on the Cyclamen alpinum, which has the ability to fight against diseases such as cancer, cardiovascular diseases, etc.

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Antioxidant, Antimicrobial, Cytotoxic, Larvicidal and Anthelmintic Activities and Phenolic Contents of Cyclamen alpinum

Synthesis, characterization, chemical and biological activities of 4-(4-methoxyphenethyl)-5- benzyl-2-hydroxy-2H-1,2,4-triazole-3(4H)-one phthalocyanine derivatives

Biosorption of Rhodamine B dye from aqueous solution by Rhus coriaria L. plant: Equilibrium, kinetic, thermodynamic and DFT calculations

Lung cancer is the most common cancer worldwide and the leading cause of cancer-related death. Plant-derived natural products and compounds are an inspiring source of chemotherapeutic agents. Alzheimer's disease (AD) is linked to the decline of acetylcholine (ACh) effects in the brain, so acetylcholinesterase (AChE) inhibitors are important in the treatment of AD. In this study, the chemical components and bioactivity of ethanolic extract of Rhabdosciadium microcalycinum (RM) was investigated by various methods. The lipophilic components of RM was determined by gas chromagraphy-mass spectrometry (GC-MS). Antioxidant activicty tests were evaluated with FCAP (Ferrous chelating antioxidant power assay), FRAP (Ferric reducing antioxidant power assay), ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt assay) and DPPH (2,2-diphenyl-1-picrylhydrazyl assay). The anticancer effect of RM was evaluated by WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate), colony formation, wound healing and CDDE (cell death detection Elisa) analysis on A549 lung cancer cells. Enzyme inhibition effects of RM was determined against AChE and α-glycosidase (AG) enzymes. According to the results of molecular docking found in the study; With a docking score of −4.56 for HP5 protein, −6.23 for BXO protein, −5.25 for AFI protein, −3.90 for gly protein and −7.74 for AChE protein, Telecinobufagin molecule was found to have higher activity than other molecules. After docking calculations, Absorption, Distribution, Metabolism, Excretion and Toxicity (ADME/T) analysis was performed to examine the effects and reactions of molecules on human metabolism. RM inhibited the viability of A549 cells dose-dependent manner (IC50=117.15±8.58 μg/mL), and it was also observed that RM suppressed colony formation, prevented cell migration and directed the cells to apoptosis. RM has important lipophilic components, and significant antioxidant and enzyme inhibition potential. IC50 values of RM for AChE and AG enzymes were found as 35.86 mg/mL, and 10.14 mg/mL, respectively. In conclusion, the findings of this study reveal that ethanol extract from the aerial part of Rhabdosciadium microcalycinum may be a potential therapeutic agent for the treatment of human lung cancer and Alzheimer's disease, due to its phytochemical components. 

The Evaluation of Anticancer, Antioxidant, Antidiabetic and Anticholinergic Potentials of Endemic
 Rhabdosciadium microcalycinum
 Supported by Molecular Docking Study

Almond is a nut that is consumed abundantly all over the world. In Turkey, almonds are grown in most parts of the Turkey and the consumption of nuts has become an essential part of healthy life. Almond contain powerful nutrients, which may be related to their fatty acid composition. Therefore, the aim of present study was to investigate the antioxidant potentials and the protective activity of ethanol extraction of Amygdalus trichamygdalus fruit parts (Hull and Drupe) against the lipid peroxidation, oxidative DNA damage and protein oxidation using soxhlet ethanol extraction. To the best of our knowledge, our study can be evaluated as the first report. The total phenolic content was found to be equivalent to 28.16 ± 9.990 μg/g gallic acid extract and the total flavonoid content was equivalent to 8.866 ± 0.208 μg/g quercetin extract. The present study shows that A. trichamygdalus extract have good protective activity against lipid peroxidation in the FeCl2–H2O2 system. The extract also showed a strong DPPH radical scavenging ability in a concentration-dependent manner. At 2000 μg/ml, the DPPH radical scavenging activity was found to be 84.47 ± 0.147%. The protein oxidation was also inhibited by A. trichamygdalus extract. At 2000  μg/ml, A. trichamygdalus extract protected protein oxidation by 76.27 ± 1.14%. In addition, the DNA oxidation damage against OH radicals resulting from H2O2 photolysis was inhibited concentration dependently and DNA damage was suppressed by 50.26 ± 2.6% at 1000  μg/ml concentration. The results suggest that A. trichamygdalus fruit extract have antioxidant activity and protect lipid, protein and DNA against oxidative damage. Therefore, the extract may be useful in the food industry as natural antioxidants.

In vitro DNA damage, protein oxidation protective activity and antioxidant potentials of almond fruit (Amygdalus trichamygdalus) parts (hull and drupe) using soxhlet ethanol extraction

Octa-substituted Zinc(II), Cu(II), and Co(II) phthalocyanines with 1-(4-hydroxyphenyl)propane-1-one: Synthesis, sensitive protonation behaviors, Ag(I) induced H-type aggregation properties, antibacterial–antioxidant activity, and molecular docking studies

Determination Of Antioxidant Activities In Freshliver (Salvia Officinalis) Plant

In this study, we determined the antioxidant activities of four different solvent fractions (ethanol, methanol, acetone and ethyl acetate) obtained from Freshliver plant leaves (Salvia officinalis) by employing two different assays such as 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) and Folin-Ciocaltaeu method. The results showed that ethanolextract of freshliver plant exhibited the highest total phenolic contents (43.55 mg GAE/g extract), followed by methanol-extract of freshliver plant (23.62 mg GAE/g extract), ethyl acetate extract (18.29 mg GAE/g extract) and acetone extract (11.58 mg GAE/g extract). All the extractions showed almost similar free radical removal activities as % inhibition of DPPH method. The values were found for methanol extract % 90.89, ethyl acetate extract, % 90.48, ethanol extract % 86.31 and acetone extract % 84.78.

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Rana Arıduru

Papers

Octa-substituted Zinc(II), Cu(II), and Co(II) phthalocyanines with 1-(4-hydroxyphenyl)propane-1-one: Synthesis, sensitive protonation behaviors, Ag(I) induced H-type aggregation properties, antibacterial–antioxidant activity, and molecular docking studies

Determination Of Antioxidant Activities In Freshliver (Salvia Officinalis) Plant

Ciğertaze Otu (Salvia Officinalis) Bitkisinin Antioksidan Aktivitesinin Belirlenmesi