Showing papers by "Richard Lathe published in 1999"
Patent•
10 Nov 1999
2 citations
TL;DR: This chapter discusses four approaches along with their merits and drawbacks, and possible means to the identification of genes and transcripts whose expression is restricted to the hippocampus, and the advantages and drawbacks of the techniques employed.
Abstract: Publisher Summary The chapter discusses four approaches along with their merits and drawbacks, and possible means to the identification of genes and transcripts whose expression is restricted to the hippocampus, and the advantages and drawbacks of the techniques employed. Unexpectedly, each approach highlights a different and non-overlapping subset of genes, but the expression of at least some of these was largely if not exclusively restricted to the hippocampus. The most important method is differential hybridization. This yielded a gene, Cyp7b, whose expression is largely but not exclusively restricted to the hippocampal formation and encoding a novel enzyme metabolizing neurosteroids. The candidate gene family approach yielded a new serine protease, BSP1 with a highly restricted pattern of expression that permits exploitation in a transgenic context, and protein tyrosine phosphatase (PTP) gamma, whose restricted pattern of expression warrants further study.
2 citations
Patent•
05 Oct 1999
TL;DR: In this paper, the production of hydroxylated and/or acetylated steroids, comprising growing yeast on a medium containing at least one precursor, where the yeast used has been transformed to express the product of the cyp7b gene, is new.
Abstract: Production (M1) of hydroxylated and/or acetylated steroids (I) comprising growing yeast on a medium containing at least one precursor (II) for (I), where the yeast used has been transformed to express the product of the cyp7b gene, is new. Independent claims are also included for the following: (1) (I) produced by M1; (2) a yeast strain lacking 17-dehydrogenase activity because of inactivation of the gene yil124w; and (3) a yeast strain transformed with a plasmid that contains a cassette for expressing the cyp7b gene.
01 Jan 1999
TL;DR: ES cells are most commonly made from mouse strains that harbour a mutation or mutations that permit the outgrowth and proliferation of cells from the early embryo that may explain, in part, irreproducibility of some transgenic experiments.
Abstract: 1. Genetic background. ES cells are most commonly made from mouse strains (such as 129) that harbour a mutation or mutations, so far unknown, that permit the outgrowth and proliferation of cells from the early embryo. ES cells are reported to have been prepared from other strains, including BL/6, but these have not been widely used. There are also potential problems of strain typing that could confound the issue (Simpson et al., 1997). Because 129 mice perform poorly on a variety of tests, transgenic mice are routinely backcrossed to a more robust strain (e.g. BL/6). This presents a problem because the control mice will tend to have the BL/6 allele (and flanking BL/6 genes) where modified or knockout mice will contain the 129 allele. This may explain, in part, irreproducibility of some transgenic experiments (Routtenberg, 1995; Gerlai, 1996; Lathe, 1997).