Predicting plasma protein binding of drugs: a new approach.

Plasma and Tissue Protein Binding of Drugs in Pharmacokinetics

High-resolution and high-throughput protocols for measuring drug/human serum albumin interactions using BIACORE.

The available techniques for the investigation of drug binding to plasma and tissues protein are reviewed and the advantages and disadvantages of the various techniques stated. A comparison of different plasma protein binding techniques is made which shows that the size of the unbound fraction of drug may be influenced by the method used. Protein binding may be assayed by methods including equilibrium dialysis, ultrafiltration, ultracentrifugation, gel filtration, binding to albumin microspheres and circular dichroism. Tissue binding techniques can involve testing binding to isolated organs, tissue slices, homogenates and isolated subcellular particles. Details of the available methods to compute pharmacokinetic constants are given. Stereoselective binding has been investigated for a limited number of drugs and the difference in the binding of 2 enantiomers is usually modest. The measurement of the binding constants is often required to characterise the drug-protein interaction. Mathematical and graphical methods to compute the pharmacokinetic parameters are discussed. The implications of binding on the volume of distribution and clearance of drugs are examined.

Methods of determining plasma and tissue binding of drugs. Pharmacokinetic consequences.

Mathematical Modelling and Computers in Endocrinology

Effect of temperature on the binding of salicylate by human serum albumin.

SummarySera from patients with chronic ulcerative colitis have low agglutination titers against a pathogenic strain of E. coli, due wholly or in part to a decreased content of 19S antibodies.

Roland F. Mais

Papers

Effect of temperature on the binding of salicylate by human serum albumin.

E. coli Agglutinating Activity of Ulcerative Colitis Sera