The Genera Aeromonas and Plesiomonas

Bile peptone broth and alkaline peptone water (pH 8.5) were examined as enrichment media for the isolation of Plesiomonas shigelloides from stools, with salmonella-shigella agar as the isolation medium. After 423 parallel examinations in two different experiments, bile peptone broth enrichment for 24 h was observed to be six times more effective (P less than 0.01) than direct plating alone on salmonella-shigella agar. Bile peptone broth was found to be twice as effective as alkaline peptone water for the recovery of P. shigelloides from stools.

Enrichment for Plesiomonas shigelloides from stools.

Aeromonas, Arcobacter, and Plesiomonas

29. Aeromonas,Plesiomonas, andArcobacter

The suitability and selectivity of nutrient broth with bile salts and varying concentrations of brilliant green as an enrichment medium was tested for the isolation of Plesiomonas shigelloides from faeces. The broth was most effective at a concentration of 0.001 g/l brilliant green, using faeces deliberately contaminated with Plesiomonas. Two hundred and nineteen faecal samples from Nigeria were then examined for the presence of Plesiomonas and other bacterial pathogens. One yielded Plesiomonas following enrichment in bile salts brilliant green broth (BBG broth) whereas no samples were positive on direct plating.

https://jcp.bmj.com/content/jclinpath/40/6/680.full.pdf

S E Millership

Papers

Isolation of Plesiomonas shigelloides from nutrient broth with brilliant green: its use in screening stool samples from an African population.