Showing papers by "Salvatore Pepe published in 2011"
TL;DR: It is demonstrated that oxidative/nitrosylative changes of actin and tropomyosin are largely increased in human failing hearts and are likely to contribute to the contractile impairment evident in end-stage HF.
141 citations
TL;DR: How to compare 'single- step' and 'multi-step' extraction processes are described and how to interpret information available through microfluidic and spectroscopic analyses to evaluate sample quality is discussed.
Abstract: High quality RNA is the key to producing meaningful gene expression analyses. Human cardiac tissue specimens are extremely valuable, but may not always be obtained under optimal conditions and are frequently fibrotic. We provide a practical guide to assist in assessing the efficacy of two different RNA extraction methods applied to these challenging specimens. We describe how to compare ‘single-step' and ‘multi-step' extraction processes and discuss how to interpret information available through microfluidic and spectroscopic analyses to evaluate sample quality.
2 citations