Samir Mounir

TL;DR: The cDNA sequence of the 3′-terminal genomic region of the Québec IAF-exp91 strain of porcine reproductive and respiratory syndrome virus (PRRSV) was determined and compared to other reference strains from Europe and US, indicating that North American PRRSV strains belong to a genotype distinct from that of the LV.

TL;DR: A neurotropism on the part of the 229E strain of human coronavirus is suggested and the need for continued studies on the possible role of coronaviruses in the etiology of MS is highlighted.

TL;DR: Results obtained by Western blotting analyses using purified virus and E. coli-expressed ORFs 5 to 7 gene products suggested that antibodies directed against the envelope E protein appear by day 7 p.i., whereas antibodies directedagainst the nucleocapsid N and membrane M proteins can only be detected by the end of the second week of PRRSV infection.

TL;DR: The Québec reference strain of PRRSV appeared to be related more closely to equine arteritis virus and lactate dehydrogenase-elevating virus than are the two European strains of the virus.

TL;DR: RT-PCR was found to be more sensitive than indirect immunofluorescence assay for detection of PRRSV in tissues from experimentally infected pigs and as sensitive as virus isolation in PAM, especially when combined with Southern blotting with the digoxigenin-labeled N probe and chemiluminescence detection.