Sandra L. Murray

TL;DR: The A. nidulans homolog (nkuA) of the human KU70 gene that is essential for nonhomologous end joining of DNA in double-strand break repair is identified andletion of nkuA (nukuAΔ) greatly reduces the frequency of nonHomologous integration of transforming DNA fragments, leading to dramatically improved gene targeting.

TL;DR: An identical core 6-bp in vitro binding site for each protein has been identified in genes encoding glyoxalate bypass, beta-oxidation, and peroxisomal functions, and northern blot analysis has shown that deletion of the farA and farB genes eliminates induction of a number of genes by both short- and long-chain fatty acids.

TL;DR: The pex mutants are able to grow on acetate but are affected in growth on fatty acids, indicating a requirement for the peroxisomal localization of β-oxidation enzymes, and mislocalization of malate synthase does not prevent growth on either fatty acids or acetate, showing that the glyoxylate cycle does not require peroxISomal localization.

TL;DR: In the filamentous ascomycete Aspergillus nidulans, tandem divergently transcribed genes (aclA and aclB) encode the subunits of ATP-citrate lyase, and these genes are deleted, indicating a specific requirement for high levels of cytoplasmic acetyl-CoA during differentiation.

TL;DR: The acuG gene encoding fructose-1,6 bisphosphatase is cloned and found that expression of this gene is regulated by carbon catabolite repression as well as by induction by a TCA cycle intermediate similar to the induction of the previously studied acuF gene encoding phosphoenolpyruvate carboxykinase.