Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.

Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions

Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.

The Purification and Mechanism of Action of Human Antithrombin-Heparin Cofactor

Publisher Summary This chapter discusses the practical methods for plasma lipoprotein analysis Changes in the concentration and sometimes in the nature of the plasma lipoproteins occur in a variety of diseases The pioneering work of Gofman and his colleagues in the early 1950s was responsible for much of the knowledge of these complex proteins that is now available Their techniques of analytical ultracentrifugation were, and still remain, the primary standard for lipoprotein analysis These methods are available in very few medical centers; however, excluding access to them by most of the laboratories where the interest and availability of patients or subjects for investigation are the greatest Recent simplifications and improvements of other analytical methods have made these a partial replacement for the analytical ultracentrifuge, and newer methods have proved their value Techniques of protein chemistry and immunology have added a new dimension to lipoprotein analysis —that of specific recognition and quantitation of the protein moieties

Scheidegger Jj

Papers

[A micro-method of immuno-electrophoresis].