Saccharomycopsis fibuligera and its applications in biotechnology

Biotechnological applications of industrially important amylase enzyme

In applied microbiology, strain improvement of microorganisms by conventional selection culture is not always successful, so single-cell selection of viable cells with the desired characteristics from a large heterogeneous population may be used instead. Single-cell selection with use of a micromanipulator is possible, but laborious. For many applications, the process has been automated. In this review, an automated method, laser scanning cytometry (LSC), is outlined together with flow cytometry (FCM). FCM is familiar to many microbiologists, but LSC is a microscopic-slide-based method that is less well known. One of its advantages is its possible use in the examination of small cell populations. In addition, individual cells can be examined repeatedly, measured automatically and later observed microscopically by the operator, and finally stored (if desired) on the microscopic slide on which they are placed. Fluorescent and other probes are available in abundance for FCM, and almost all might be used in LSC. A number of applications of these methods are cited from the extensive literature (mostly about FCM), but the list of possible applications in this review is far from being exhaustive. This review is intended as an introduction for the applied microbiologist to the manifold uses of LSC.

Single‐Cell Sorting of Microorganisms by Flow or Slide‐Based (Including Laser Scanning) Cytometry

The advancement of biological weed control is limited by the slow development of effective, broader-spectrum biological control agents. Protoplast fusion was carried out between the Helminthosporium gramineum subsp. echinochloae (HGE) strain, HM1, and Curvularia lunata (CL) to breed new strains with improved biocontrol efficiency. The HM1 strain was derived from HGE by ultraviolet (UV) treatment. Conditions for protoplast fusion were optimized, including lytic enzyme mixtures and incubation time. The efficacy of lytic enzyme mixtures on cell wall digestion was also compared. The most effective lytic enzyme mixture for CL was 2% lywallzyme plus 2% snailase and for HM1, 2% cellulase plus 2% snailase. The optimum incubation time was 16 h for CL and 24 h for HM1. All fusant strains exhibited similar morphological and conidial properties to the HM1 parent. A total of 1,360 fusant strains were produced, 136 of which were randomly selected for characterization. Seven fusant strains showed improved spore...

Biological Control Agent for Rice Weeds from Protoplast Fusion between Curvularia lunata and Helminthosporium gramineum

https://www.scielo.br/pdf/bjm/v41n1/v41n1a35.pdf

Seong Hyun Choi

Papers

Intergeneric protoplast fusion in Saccharomycopsis fibuligera and Saccharomyces cerevisiae