FIELD: chemistrySUBSTANCE: invention describes a method of determining concentration or content of ascorbic acid in liquid drug formations, involving preparation of doses of an analysed sample and a standard sample, wherein 06 ml of a sodium phosphate buffer (005 mol/l, pH 8,3), 01 ml of tetramethylene diamine solution (005 mol/l) in a sodium salt of ethylenediamine tetraacetic acid (02 mmol/l), 01 ml of the analysed sample and 02 ml of riboflavin solution (0034 mmol/l) are successively fed into a chemiluminometer cell; the same volume of distilled water is added to the control sample; further, the reaction is initiated by irradiation with a visible light lamp (100 W) at a distance of 20 cm for 60 s; 10 s before irradiation time expires, 01 ml of luminol solution (025 mmol/l) is added; after irradiation, the cell holder is moved into the operating position in front of the photocathode of a photomultiplier (about 1 s) in a biochemiluminometer and the chemiluminometer flash is detected; concentration of ascorbic acid in the mixture or content thereof is calculated in the same units it was recorded using a formulaEFFECT: method is characterised by optimum rapidness, easy preparation of samples for analysis and high reproducibility and accuracy4 ex, 1 tbl, 1 dwg

Method of determining concentration of ascorbic acid

FIELD: chemistry. ^ SUBSTANCE: invention relates to analytic chemistry of organic compounds concerning elaboration of processes of vitamin isolation and separation. Method includes preparation of water-salt solution of ascorbic acid by its dissolving in saturated desalter solution, extraction of ascorbic acid, analysis of equilibrium water phase, as desalter for preparation of water-salt solution of ascorbic acid, saturated solution of ammonium sulphate or sodium sulphite is used; water-salt solution of ascorbic acid is prepared with pH 5, for which purpose weighed portion of ascorbic acid with weight 1 mg, measured on analytic scales, is carried to measuring flask, which has volume 100 ml, and bring to the mark with saturated solution of ammonium sulphate or sodium sulphite, extraction of ascorbic acid is carried out by water solution of polymer poly-N-vinylpyrrolidone with concentration 10-5 mole/l with ratio of polymer solution and water-salt solution volumes 1:5. ^ EFFECT: increased degree of ascorbic acid extraction from water solution and increase of analysis safety. ^ 2 cl, 1 tbl

Shatalov Gennadij Valentinovic

Papers

Method of detecting ascorbic acid in water solution