Showing papers by "Shuxun Yu published in 2023"
TL;DR: In this article , GhGAI was used as a bait protein to screen a cDNA library for interacting proteins related to flowering in Arabidopsis thaliana, and the results of yeast two-hybrid, bimolecular fluorescence complementation (BiFC) and coimmunoprecipitation (Co-IP) assays showed that Gh GAI interacted with GhAP1.
TL;DR: In this paper , an expression analysis showed that GhCYP94C1 was highly expressed in the roots and was suppressed by drought treatment, which indicated that the gene may play an important role in drought resistance.
Abstract: Cytochrome P450 proteins belong to one of the largest families of enzyme proteins in plants and play important roles in plant growth and development and the stress response. In our previous studies, a cytochrome P450 gene, GhCYP94C1 (cytochrome P450 94C1), was functionally characterized as a positive regulator of seed germination, main root elongation and early flowering. However, whether the gene has other potential functions remains to be further explored. In our study, expression analysis showed that GhCYP94C1 was highly expressed in roots and was suppressed by drought treatment. Endogenous silencing of GhCYP94C1 via virus-induced gene silencing (VIGS) increased drought resistance in cotton plants, which was accompanied by the upregulated expression of the abscisic acid (ABA) biosynthesis gene nine-cis-epoxycarotenoid dioxygenase 9 (GhNCED9) during drought stress. Our findings suggested that GhCYP94C1 may play an important role in drought resistance. Combined with previous research results, the present results provide a theoretical basis for future breeding of new cotton varieties with early maturation and drought resistance.