Sidney E. Grossberg

TL;DR: An assay for proteins in solution that depends on the conversion of Coomassie brilliant blue G250 in dilute acid from a brownish-orange to an intense blue color has high reproducibility and can detect less than 1.0 μg of albumin.

TL;DR: Two-dimensional gel electrophoresis demonstrates the existence of a set of polypeptides whose synthesis is uniquely induced by IFN-γ, which has the same broad range of biochemical and biological actions as do IFn-α andIFN-β, although relative potencies vary depending on the cell type and function investigated.

TL;DR: Because interferon can alter the program of events involved in conversion of 3T3-L1 fibroblasts into adipose cells, it may be able to affect the regulation of eukaryotic cell differentiation.

TL;DR: The present results support the recommendation that the preferred way to state the index of neutralization of antibodies is a titer (t), calculated by the formula t = f(n - 1)/9, where f is the reciprocal of the antibody dilution achieving the end point, and n is the IFN concentration measured in that day's titration.

TL;DR: The new MxA gene-induction bioassay is adapted to measure neutralizing antibodies to interferon- beta1b (IFN-beta1b, the active ingredient in Betaseron) in sera from patients treated with Betaserson, and validated to quantify neutralizing titers of 1:20 and above.