Showing papers by "Simon G. Gregory published in 1998"

7 – Sequence Ready Clone Maps

Abstract: Rapid progress in determination of the DNA sequence of the human genome depends on the availability of bacterial clones ready to sequence. This is best achieved through the rapid production of accurate bacterial clone maps, and the selection of the optimal minimal tiling set of clones from these maps. The properties required of the “sequence ready” clone map include several-fold coverage, construction of contigs with a method that allows integration of clones from different libraries, a unique name for each clone and a set of experimental data attached to that name, at least some of which can be linked directly to its sequence, anchoring of the map to genome or chromosome-wide long-range maps in order to coordinate within the project and with the rest of the world. This chapter describes an integrated strategy for construction of sequence ready clone maps, and a suite of software packages, which can be integrated to provide a system suitable for construction of sequence ready contigs in any genome project. It describes how the components can be linked together to enable effective coordination of the different stages of the strategy.