Single molecule array-based sequencing permits simultaneous analysis of potentially hundreds of millions of single molecules of DNA per cm2 in an array-based format. In this massively parallel approach to DNA sequence acquisition, reagents are utilized in an extremely efficient manner to achieve unprecedented levels of cost reduction and increased throughput. Ultimately, single molecule array (SMA)-based sequencing will enable analysis of individual whole human genomes in a single experiment.


Keywords:

sequencing;
resequencing;
whole-genome sequencing;
single molecule array;
clustered arrays;
sequencing chemistry;
fluorescence detection;
SNP detection;
genotyping;
genome variation

Simon T. Bennett

Papers

Single molecule array‐based sequencing