Sofia Loupassaki

TL;DR: Using the developed molecular tools, new yeast strains were generated with increased capacity to produce plant terpenoids, and the durability of the strains allow successive rounds of improvement to maximize yields.

TL;DR: The modular structure of geranylgeranyl diphosphate synthesis in yeast is identified to be a major limitation in diterpene yields, and the Erg20p is engineer, which supports efficient isoprenoid production and can be used as a dedicated chassis for diterPene production or biosynthetic pathway elucidation.

TL;DR: This work highlights the usefulness of synthetic biology approaches in the elucidation of plant natural product biosynthetic pathways and develops yeast as a tool to exploit the wealth of next-generation sequencing data that are constantly being produced through transcriptomic, genomic, or metagenomic studies.

TL;DR: A modular platform based on standardized and exchangeable parts is developed to reproduce and potentially expand the diversity of terpene structures in Saccharomyces cerevisiae and provides a platform on which additional gene mining, combinatorial biosynthesis, and protein engineering efforts can be integrated to sustainably explore the terPene chemical space.

TL;DR: The aim of the present work was to study the encapsulation of Origanum onites L. essential oil (oregano EO) in β-cyclodextrin (β-CD) inclusion complexes (ICs), using the co-precipitation method, and to determine the inclusion efficacy and the study of the encapsulated oregana EO release profile.