Showing papers by "Stacie M. Jones published in 1997"

Evidence for alternative splicing in hepatic alpha 1B-adrenergic receptor gene expression.

Abstract: The interactions of catecholamines with α1B-adrenergic receptors (α1B-AR) located on the surface of many cell types are responsible for physiologic and pathologic functions in mammalian systems. Transcription of the α1B-AR gene leads to the expression of multiple α1B-AR mRNAs that are distributed in a tissue-specific fashion. The purpose of this study was to define the 5′-untranslated regions of the multiple α1B-AR gene transcripts. Evidence for a previously unidentified intron in the α1B-AR gene upstream of the receptor open reading frame was obtained via rapid amplification of cDNA ends. A product was amplified and was found to be missing the nucleotide interval from -708 to -194, (+1 is the start of translation). Evidence for tissue-specific alternative intron splicing was obtained from ribonuclease protection assays and RT-PCR experiments. Using an RNA probe extending from -240 to +93 and including 45 nucleotides into the putative intron, a single protected fragment was detected in heart RNA w...