Showing papers by "Stefan de Folter published in 2023"
TL;DR: In this paper , the bHLH transcription factor SPATULA (SPT) has been identified as a regulator during different stages of Arabidopsis development, including the control of leaf size.
Abstract: The bHLH transcription factor SPATULA (SPT) has been identified as a regulator during different stages of Arabidopsis development, including the control of leaf size. However, the mechanism via which it performs this function has not been elucidated. To better understand the role of SPT during leaf development, we used a transcriptomic approach to identify putative target genes. We found putative SPT target genes related to leaf development, and to stomata and trichome formation. Furthermore, genes related to anthocyanin biosynthesis. In this work, we demonstrate that SPT is a negative regulator of stomata number and a positive regulator of trichome number. In addition, SPT is required for sucrose-mediated anthocyanin biosynthesis.
1 citations
TL;DR: In this paper , the authors combine quantitative live-imaging at cellular resolution with genetics, chemical treatments, and modeling to understand the formation of Arabidopsis thaliana female reproductive organ (gynoecium).
Abstract: Morphogenesis requires the coordination of cellular behaviors along developmental axes1. In plants, gradients of growth and differentiation are typically established along a single longitudinal primordium axis to control organ shaping2. Here we combine quantitative live-imaging at cellular resolution with genetics, chemical treatments, and modeling to understand the formation of Arabidopsis thaliana female reproductive organ (gynoecium). We show that, contrary to other aerial organs, gynoecium shape is determined by two competing differentiation gradients positioned along two orthogonal axes. An early mediolateral gradient, dependent on meristematic activity in the medial domain, controls the valve morphogenesis while simultaneously restricting an auxin-dependent, longitudinal gradient to the style. This gradient competition serves to finetune the common developmental program governing organ morphogenesis to ensure the specialized function of the gynoecium3,4.
1 citations
TL;DR: In this paper , the authors test the hypothesis that epigenome editing via CRISPR activation (CRISPRa) activate tomato plant defense genes to confer resistance against pathogen attack.
1 citations
TL;DR: In this paper , a review of the existing knowledge about the evolution, development and molecular mechanisms involved in the origin and evolution of the gynoecium is presented, along with a discussion of the extent of the conservation of the molecular mechanisms for gynocyte development among different taxa, and how these mechanisms give origin and diversification to the gynaecium.
Abstract: The appearance of the flower marks a key event in the evolutionary history of plants. Among the four types of floral organs, the gynoecium represents the major adaptive advantage of the flower. The gynoecium is an enclosing structure that protects and facilitates the fertilisation of the ovules, which then mature as seeds. Upon fertilisation, in many species, the gynoecium itself eventually becomes the fruit, which contributes to the dispersal of the seeds. However, despite its importance and the recent advances in our understanding of the genetic regulatory network (GRN) guiding early gynoecium development, many questions remain to be resolved regarding the extent of the conservation of the molecular mechanisms for gynoecium development among different taxa, and how these mechanisms give origin and diversification to the gynoecium. In this review, we compile the existing knowledge about the evolution, development and molecular mechanisms involved in the origin and evolution of the gynoecium.