S
Stefan Minning
Researcher at University of Stuttgart
Publications - 6
Citations - 292
Stefan Minning is an academic researcher from University of Stuttgart. The author has contributed to research in topics: Rhizopus oryzae & Rhizopus. The author has an hindex of 4, co-authored 4 publications receiving 287 citations.
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Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
TL;DR: The lipases of the Rhizopus species family are important and versatile enzymes that are mainly used in fat and oil modification due to their strong 1,3-regiospecificity and could be further increased by introducing a transition phase that involved the simultaneous feeding of glycerol and methanol followed by a single meethanol feed.
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Functional expression of Rhizopus oryzae lipase in Pichia pastoris: high-level production and some properties
TL;DR: Recombinant ROL lipase was purified to homogeneity by a simple two-step purification procedure and had a specific activity of 8571 U mg-1 (triolein, 30 degrees C, pH 8.1) which is comparable with the purified native enzyme.
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Designing new metal affinity peptides by random mutagenesis of a natural metal-binding site
TL;DR: The metal-binding site of a Helicobacter pylori ATPase 439 (heli(WT)-tag) was successfully used as a new fusion peptide for immobilized metal ion affinity chromatography (IMAC) and produced higher yields than the frequently used his6-tag.
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Determination of peracid and putative enzymatic peracid formation by an easy colorimetric assay
TL;DR: In this paper, a semi-quantitative colorimetric method for the determination of peracids in the presence of hydrogen peroxide in Luria-Bertani agar is described.
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MIT CAACB Risk Assessment Case Study: Assessing Virus Cross-Contamination Risk between Two Simultaneous Processes in an Open Biomanufacturing Facility
Andrea L. Koenigsberg,Veronica Fowler,Reuben Domike,Audrey Brussel,Paul W. Barone,Flora J. Keumurian,James C. Leung,Michael E. Wiebe,Michael T Brewer,Shawn Chan,Nicolas Dumey,Anne Fournillier,Marcella Goodnight,Johanna Kindermann,Richard Leavy,Buyoung Lee,Stefan Minning,Marie Murphy,Eric R. Myers,Armen Nahabedian,Kavita Nanda,Sandi K. Parriott,G. K. Raju,Ciaran Scallan,S. Schoch,Joe Shiminsky,Bonnie Shum,Sebastian Teitz,Bernice Westrek,Stacy L. Springs +29 more
TL;DR: In this article , the authors conducted a case study exercise to assess the risks of cross-contamination between a human and non-human host cell system in an open manufacturing facility.