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Stephen Scaringe
Researcher at Dharmacon, Inc.
Publications - 118
Citations - 4418
Stephen Scaringe is an academic researcher from Dharmacon, Inc.. The author has contributed to research in topics: RNA & Trans-acting siRNA. The author has an hindex of 23, co-authored 118 publications receiving 4314 citations. Previous affiliations of Stephen Scaringe include Thermo Fisher Scientific & Wayne State University.
Papers
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Journal ArticleDOI
Rational siRNA design for RNA interference
Angela Reynolds,Devin Leake,Queta Boese,Stephen Scaringe,William Marshall,Anastasia Khvorova +5 more
TL;DR: Application of an algorithm incorporating all eight characteristics associated with siRNA functionality significantly improves potent siRNA selection and highlights the utility of rational design for selecting potent siRNAs and facilitating functional gene knockdown studies.
Patent
Methods and compositions for selecting siRNA of improved functionality
Anastasia Khvorova,Angela Reynolds,Devin Leake,William Marshall,Steven Read,Stephen Scaringe +5 more
TL;DR: In this paper, a rational design of siRNAs for gene silencing is described, including those directed to the nucleotide sequences for HAO1, and methods for silencing genes.
Journal ArticleDOI
A library of siRNA duplexes targeting the phosphoinositide 3‐kinase pathway: determinants of gene silencing for use in cell‐based screens
Andrew C. Hsieh,Ronghai Bo,Judith Manola,Francisca Vazquez,Olivia Bare,Anastasia Khvorova,Stephen Scaringe,William R. Sellers +7 more
TL;DR: Analysis of the parameters correlating with effective knockdown showed that among duplexes that achieved a >70% knockdown of the mRNA there were strong nucleotide preferences at specific positions, most notably positions 11 (G or C) and 19 (T) of the siRNA duplex.
Journal ArticleDOI
RNA oligonucleotide synthesis via 5'-silyl-2'-orthoester chemistry.
TL;DR: A new approach for RNA synthesis that is now as reliable and efficient as DNA synthesis methods is described in this report, which has enabled the routine high-quality synthesis of RNA oligonucleotides up to 50 bases in length regardless of sequence or secondary structure.
Journal ArticleDOI
An RNA ligase-mediated method for the efficient creation of large, synthetic RNAs
TL;DR: The ability to ligate synthetic or in vitro transcribed RNA with high efficiency has the potential to open up areas of RNA biology to new functional and biophysical investigation and it is anticipated that site-specific incorporation of fluorescent dyes into large RNA molecules will yield a wealth of new information on RNA structure and function.