The influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition. 
The isotopic composition of the nitrogen in an animal reflects the nitrogen isotopic composition of its diet. The δ^(15)N values of the whole bodies of animals are usually more positive than those of their diets. Different individuals of a species raised on the same diet can have significantly different δ^(15)N values. The variability of the relationship between the δ^(15)N values of animals and their diets is greater for different species raised on the same diet than for the same species raised on different diets. Different tissues of mice are also enriched in ^(15)N relative to the diet, with the difference between the δ^(15)N values of a tissue and the diet depending on both the kind of tissue and the diet involved. The δ^(15)N values of collagen and chitin, biochemical components that are often preserved in fossil animal remains, are also related to the δ^(15)N value of the diet. 
The dependence of the δ^(15)N values of whole animals and their tissues and biochemical components on the δ^(15)N value of diet indicates that the isotopic composition of animal nitrogen can be used to obtain information about an animal's diet if its potential food sources had different δ^(15)N values. The nitrogen isotopic method of dietary analysis probably can be used to estimate the relative use of legumes vs non-legumes or of aquatic vs terrestrial organisms as food sources for extant and fossil animals. However, the method probably will not be applicable in those modern ecosystems in which the use of chemical fertilizers has influenced the distribution of nitrogen isotopes in food sources. 
The isotopic method of dietary analysis was used to reconstruct changes in the diet of the human population that occupied the Tehuacan Valley of Mexico over a 7000 yr span. Variations in the δ^(15)C and δ^(15)N values of bone collagen suggest that C_4 and/or CAM plants (presumably mostly corn) and legumes (presumably mostly beans) were introduced into the diet much earlier than suggested by conventional archaeological analysis.

Influence of Diet On the Distribtion of Nitrogen Isotopes in Animals

Worldwide, nitrogen use efficiency (NUE) for cereal production (wheat, Triticum aestivum L.; corn, Zea mays L.; rice, Oryza sativa L. and O. glaberrima Steud.; barley, Hordeum vulgare L.; sorghum, Sorghum bicolor (L.) Moench; millet, Pennisetum glaucum (L.) R. Br.; oat, Avena sativa L.; and rye, Secale cereale L.) is approximately 33%. The unaccounted 67% represents a $15.9 billion annual loss of N fertilizer (assuming fertilizer-soil equilibrium). Loss of fertilizer N results from gaseous plant emission, soil denitrification, surface runoff, volatilization, and leaching. Increased cereal NUE is unlikely, unless a systems approach is implemented that uses varieties with high harvest index, incorporated NH₄-N fertilizer, application of prescribed rates consistent with in-field variability using sensor-based systems within production fields, low N rates applied at flowering, and forage production systems. Furthermore, increased cereal NUE must accompany increased yields needed to feed a growing world population that has yet to benefit from the promise of N₂-fixing cereal crops. The Consultative Group on International Agricultural Research (CGIAR) linked with advanced research programs at universities and research institutes is uniquely positioned to refine fertilizer N use in the world via the extension of improved NUE hybrids and cultivars and management practices in both the developed and developing world. Contribution from the Okla. Agric. Exp. Stn.

https://nue.okstate.edu/Index_Publications/Improving_NUE.pdf

Improving Nitrogen Use Efficiency for Cereal Production

We report a new method for measurement of the isotopic composition of nitrate (NO3-) at the natural-abundance level in both seawater and freshwater. The method is based on the isotopic analysis of nitrous oxide (N2O) generated from nitrate by denitrifying bacteria that lack N2O-reductase activity. The isotopic composition of both nitrogen and oxygen from nitrate are accessible in this way. In this first of two companion manuscripts, we describe the basic protocol and results for the nitrogen isotopes. The precision of the method is better than 0.2‰ (1 SD) at concentrations of nitrate down to 1 μM, and the nitrogen isotopic differences among various standards and samples are accurately reproduced. For samples with 1 μM nitrate or more, the blank of the method is less than 10% of the signal size, and various approaches may reduce it further.

A Bacterial Method for the Nitrogen Isotopic Analysis of Nitrate in Seawater and Freshwater

Seagrass meadows are some of the most productive ecosystems on Earth. An analysis of organic carbon data from just under one thousand seagrass meadows indicates that, globally, these systems could store between 4.2 and 8.4 Pg carbon.

Seagrass ecosystems as a globally significant carbon stock

We report a novel method for measurement of the oxygen isotopic composition (18O/16O) of nitrate (NO3-) from both seawater and freshwater. The denitrifier method, based on the isotope ratio analysis of nitrous oxide generated from sample nitrate by cultured denitrifying bacteria, has been described elsewhere for its use in nitrogen isotope ratio (15N/14N) analysis of nitrate.1 Here, we address the additional issues associated with 18O/16O analysis of nitrate by this approach, which include (1) the oxygen isotopic difference between the nitrate sample and the N2O analyte due to isotopic fractionation associated with the loss of oxygen atoms from nitrate and (2) the exchange of oxygen atoms with water during the conversion of nitrate to N2O. Experiments with 18O-labeled water indicate that water exchange contributes less than 10%, and frequently less than 3%, of the oxygen atoms in the N2O product for Pseudomonas aureofaciens. In addition, both oxygen isotope fractionation and oxygen atom exchange are consi...

Measurement of the Oxygen Isotopic Composition of Nitrate in Seawater and Freshwater Using the Denitrifier Method

A new method for collection of nitrate from fresh water and the analysis of nitrogen and oxygen isotope ratios

Riverine particulate organic matter (POM) samples were collected bi-weekly to monthly from 40 sites in the Mississippi, Colorado, Rio Grande, and Columbia River Basins (USA) in 1996–97 and analysed for carbon and nitrogen stable isotopic compositions. These isotopic compositions and C : N ratios were used to identify four endmember sources of POM: plankton, fresh terrestrial plant material, aquatic plants, and soil organic material. This large-scale study also incorporated ancillary chemical and hydrologic data to refine and extend the interpretations of POM sources beyond the source characterizations that could be done solely with isotopic and elemental ratios. The ancillary data were especially useful for differentiating between seasonal changes in POM source materials and the effects of local nutrient sources and in-stream biogeochemical processes.



Average values of δ13C and C : N for all four river systems suggested that plankton is the dominant source of POM in these rivers, with higher percentages of plankton downstream of reservoirs. Although the temporal patterns in some rivers are complex, the low δ13C and C : N values in spring and summer probably indicate plankton blooms, whereas relatively elevated values in fall and winter are consistent with greater proportions of decaying aquatic vegetation and/or terrestrial material. Seasonal shifts in the δ13C of POM when the C : N remains relatively constant probably indicate changes in the relative rates of photosynthesis and respiration. Periodic inputs of plant detritus are suggested by C : N ratios >15, principally on the Columbia and Ohio Rivers. The δ15N and δ13C also reflect the importance of internal and external sources of dissolved carbon and nitrogen, and the degree of in-stream processing. Elevated δ15N values at some sites probably reflect inputs from sewage and/or animal waste. This information on the spatial and temporal variation in sources of POM in four major river systems should prove useful in future food web and nutrient transport studies. Published in 2001 by John Wiley & Sons, Ltd.

Carbon and nitrogen isotopic compositions of particulate organic matter in four large river systems across the United States

A study was conducted to determine whether NO3 stable isotopes (δ15N and δ18O), at natural abundance levels, could discriminate among NO3 sources from sites with different land uses at the basin scale. Water samples were collected from 24 sites in the Mississippi River Basin from five land-use categories: (1) large river basins (>34 590 km2) draining multiple land uses and smaller basins in which the predominant land use was (2) urban, (3) undeveloped, (4) crops, or (5) crops and livestock. Our data suggest that riverine nitrates from different land uses have overlapping but moderately distinct isotopic signatures. δ18O data were critical in showing abrupt changes in NO3 source with discharge. The isotopic values of large rivers resembled crop sites, sites with livestock tended to have δ15N values characteristic of manure, and urban sites tended to have high δ18O values characteristic of atmospheric nitrate.

http://snobear.colorado.edu/Markw/WatershedBio/Nitrogen/Isotopes_N/kendall_2002.pdf

Nitrate stable isotopes : Tools for determining nitrate sources among different land uses in the Mississippi River Basin

Linking hyporheic flow and nitrogen cycling near the Willamette River: A large river in Oregon, USA

Recently, methods have been developed to analyze NO3- for δ15N and δ18O, improving our ability to identify NO3- sources and transformations. However, none of the existing methods are suited for wat...

Steven R. Silva

Papers

A new method for collection of nitrate from fresh water and the analysis of nitrogen and oxygen isotope ratios

Carbon and nitrogen isotopic compositions of particulate organic matter in four large river systems across the United States

Nitrate stable isotopes : Tools for determining nitrate sources among different land uses in the Mississippi River Basin

Linking hyporheic flow and nitrogen cycling near the Willamette River: A large river in Oregon, USA

A method for nitrate collection for δ15N and δ18O analysis from waters with low nitrate concentrations