The invention discloses a production method of a Relinqing preparation. The Relinqing preparation is prepared from Polygonum capitatum and auxiliary materials. According to properties of various medicinal materials in a formula of the Relinqing preparation as well as roles of the medicinal materials in the formula, the specific production method and parameters of the Relinqing preparation are improved, so that gallic acid used as an effective ingredient of the Polygonum capitatum can be detected easily, and the content of the measured gallic acid is high. Thus, the preparation is more refined and has the more significant efficacy and the good clinical effect.

Production method of Relinqing preparation

The invention provides a preparation method of polygonum capitatum flavonoid glycoside compounds. The preparation method includes the steps that polygonum capitatum is smashed, methanol extraction is carried out, suction filtration concentration is carried out, ethyl acetate concentration is carried out after water and ethyl acetate solvent distribution, separation is carried out with a silica column, then purification is carried out with an ODS-bonded silica filler open column and HPLC, and the polygonum capitatum flavonoid glycoside compounds are obtained. It is shown through a brewer's yeast K6001 bioactivity system that all the four flavonoid glycoside compounds can significantly prolong the replicative life of yeast. The polygonum capitatum flavonoid glycoside compounds can be applied to preparation of anti-aging drugs or health-care products. The preparation method is simple, and the extracted product is high in purity; polygonum capitatum is a cheap traditional Chinese medicinal material easy to obtain, the pharmaceutical application of polygonum capitatum is increased, and the anti-aging potential of the components of polygonum capitatum is found.

Preparation and anti-aging application of polygonum capitatum flavonoid glycoside compounds

The invention relates to a method for purifying silibinin. The method comprises the following steps: dissolving a silibinin crude product into ethyl acetate so as to enable the silibinin crude productto be dissolved; adding silica gel into an obtained solution, and concentrating under the reduced pressure so as to enable the ethyl acetate in the solution to be completely volatilized; adding an obtained sample into a silica gel column, carrying out gradient elution by using chloroform-methanol, and detecting by using a thin-layer chromatography; concentrating an obtained eluant, and drying toobtain a product. The technology provided by the invention is simple and convenient to operate, easy to implement and mild in conditions; a solvent used for purifying is low in toxicity, and the product purity reaches 99.5% or above; the method solves the problems that the product obtained by using existing technology is lower in purity and more in impurities.

Sun Lianna

Papers

Method for extracting and preparing high-purity silibinin from polygonum capitatum herb