Heavy metals in soils

In addition to the often-cited advantages of using Arabidopsis thaliana as a model system in plant biological research (1), Arabidopsis has many additional characteristics that make it an attractive experimental organism for studying lea d (Pb) accumulation and tolerance in plants. These include its fortuitous familial relationship to many known metal hyperaccumulators (Brassicaceae), as well as similar Pbaccumulation patterns to most other plants. Using nutrient-agar plates, hydroponic culture, and Pb-contaminated soils as growth media, we found significant variation in Arabidopsis thaliana ecotypes in accumulation and tolerance of Pb. In addition, we have found that Pb accumulation is not obligatorily linked with Pb tolerance, suggesti ng that different genetic factors control these two processes. We also screened ethyl methanesulfonate-mutagenized M2 populations and identified several Pb-accumulating mutants. Current characterization of these mutants indicates that their phenotypes are likely due to alteration of general metal ion uptake or translocation processes since these mutants also accumulate many other metals in shoots. We expect that further characterization of the ecotypes and mutants will shed light on the basic genetic and physiological underpinnings of plant-based Pb remediation. 7. Aromatic nitroreduction of acifluorfen in soils, rhizospheres, and pure cultures of rhizobacteria. Zablotowicz, R. M., Locke, M. A., and Hoagland, R. E. Phytoremediation of soil and water contaminants. Washington, DC : American Chemical Society, 1997. p. 38-53. NAL Call #: QD1.A45-no.664 Abstract: Reduction of nitroaromatic compounds to their corresponding amino derivatives is one of several pathways in the degradation of nitroxenobiotics. Our studies with the nitrodiphenyl ether herbicide acifluorfen showed rapid metabolism to am inoacifluorfen followed by incorporation into unextractable soil components in both soil and rhizosphere suspensions. Aminoacifluorfen was formed more rapidly in rhizospheres compared to soil, which can be attributed to higher microbial populations, espec ially of Gram-negative bacteria. We identified several strains of Pseudomonas fluorescens that possess nitroreductase activity capable of converting acifluorfen to aminoacifluorfen. Factors affecting acifluorfen nitroreductase activity in pure cultures an d cell-free extracts, and other catabolic transformations of acifluorfen, ether bond cleavage, are discussed. Plant rhizospheres should be conducive for aromatic nitroreduction. Nitroreduction by rhizobacteria is an important catabolic pathway for the ini tial degradation of various nitroherbicides and other nitroaromatic compounds in soils under Reduction of nitroaromatic compounds to their corresponding amino derivatives is one of several pathways in the degradation of nitroxenobiotics. Our studies with the nitrodiphenyl ether herbicide acifluorfen showed rapid metabolism to am inoacifluorfen followed by incorporation into unextractable soil components in both soil and rhizosphere suspensions. Aminoacifluorfen was formed more rapidly in rhizospheres compared to soil, which can be attributed to higher microbial populations, espec ially of Gram-negative bacteria. We identified several strains of Pseudomonas fluorescens that possess nitroreductase activity capable of converting acifluorfen to aminoacifluorfen. Factors affecting acifluorfen nitroreductase activity in pure cultures an d cell-free extracts, and other catabolic transformations of acifluorfen, ether bond cleavage, are discussed. Plant rhizospheres should be conducive for aromatic nitroreduction. Nitroreduction by rhizobacteria is an important catabolic pathway for the ini tial degradation of various nitroherbicides and other nitroaromatic compounds in soils under phytoremediation management. 8. Ascorbate: a biomarker of herbicide stress in wetland plants. Lytle, T. F. and Lytle, J. S. Phytoremediation of soil and water contaminants. Washington, DC : American Chemical Society, 1997. p. 106-113. NAL Call #: QD1.A45-no.664 Abstract: In laboratory exposures of wetland plants to low herbicide levels (<0.1 micrograms/mL), some plants showed increased total ascorbic acid suggesting a stimulatory effect on ascorbic acid synthesis occurred; at higher herbicide conce ntrations (greater than or equal to 0.1 micrograms/mL) a notable decline in total ascorbic acid and increase in the oxidized form, dehydroascorbic acid occurred. Vigna luteola and Sesbania vesicaria were exposed for 7 and 21 days respectively to atrazine (0.05 to 1 microgram/mL); Spartina alterniflora 28 days at 0.1 micrograms/mL trifluralin; Hibiscus moscheutos 14 days at 0.1 and 1 microgram/mL metolachlor in fresh and brackish water. The greatest increase following low dosage occurred with S. alterniflo ra, increasing from <600 micrograms/g wet wt. total ascorbic acid to >1000 micrograms/g. Ascorbic acid may be a promising biomarker of estuarine plants exposed to herbicide runoff; stimulation of ascorbic acid synthesis may enable some wetland plant s used in phytoremediation to cope with low levels of these compounds. In laboratory exposures of wetland plants to low herbicide levels (<0.1 micrograms/mL), some plants showed increased total ascorbic acid suggesting a stimulatory effect on ascorbic acid synthesis occurred; at higher herbicide conce ntrations (greater than or equal to 0.1 micrograms/mL) a notable decline in total ascorbic acid and increase in the oxidized form, dehydroascorbic acid occurred. Vigna luteola and Sesbania vesicaria were exposed for 7 and 21 days respectively to atrazine (0.05 to 1 microgram/mL); Spartina alterniflora 28 days at 0.1 micrograms/mL trifluralin; Hibiscus moscheutos 14 days at 0.1 and 1 microgram/mL metolachlor in fresh and brackish water. The greatest increase following low dosage occurred with S. alterniflo ra, increasing from <600 micrograms/g wet wt. total ascorbic acid to >1000 micrograms/g. Ascorbic acid may be a promising biomarker of estuarine plants exposed to herbicide runoff; stimulation of ascorbic acid synthesis may enable some wetland plant s used in phytoremediation to cope with low levels of these compounds. 9. Atmospheric nitrogenous compounds and ozone--is NO(x) fixation by plants a possible solution. Wellburn, A. R. New phytol. 139: 1 pp. 5-9. (May 1998). NAL Call #: 450-N42 Descriptors: ozoneair-pollution nitrogen-dioxide nitric-oxide air-quality tolerancebioremediationacclimatizationnutrient-sources nutrient-uptake plantscultivarsgenetic-variation literature-reviews 10. Atrazine degradation in pesticide-contaminated soils: phytoremediation potential. Kruger, E. L., Anhalt, J. C., Sorenson, D., Nelson, B., Chouhy, A. L., Anderson, T. A., and Coats, J. R. Phytoremediation of soil and water contaminants. Washington, DC : American Chemical Society, 1997. p. 54-64. NAL Call #: QD1.A45-no. 664 Abstract: Studies were conducted in the laboratory to determine the fate of atrazine in pesticide-contaminated soils from agrochemical dealer sites. No significant differences in atrazine concentrations occurred in soils treated with atrazine i ndividually or combinations with metolachlor and trifluralin. In a screening study carried out in soils from four agrochemical dealer sites, rapid mineralization of atrazine occurred in three out of eight soils tested, with the greatest amount occurring i n Bravo rhizosphere soil (35% of the applied atrazine after 9 weeks). Suppression of atrazine mineralization in the Bravo rhizosphere soil did not occur with the addition of high concentrations of herbicide mixtures, but instead was increased. Plants had a positive impact on dissipation of aged Studies were conducted in the laboratory to determine the fate of atrazine in pesticide-contaminated soils from agrochemical dealer sites. No significant differences in atrazine concentrations occurred in soils treated with atrazine i ndividually or combinations with metolachlor and trifluralin. In a screening study carried out in soils from four agrochemical dealer sites, rapid mineralization of atrazine occurred in three out of eight soils tested, with the greatest amount occurring i n Bravo rhizosphere soil (35% of the applied atrazine after 9 weeks). Suppression of atrazine mineralization in the Bravo rhizosphere soil did not occur with the addition of high concentrations of herbicide mixtures, but instead was increased. Plants had a positive impact on dissipation of aged atrazine in soil, with significantly less atrazine extractable from Kochia-vegetated soils than from nonvegetated soils. 11. Bacterial inoculants of forage grasses that enhance degradation of 2-chlorobenzoic acid in soil. Siciliano, S. D. and Germida, J. J. Environ toxicol chem. 16: 6 pp. 1098-1104. (June 1997). NAL Call #: QH545.A1E58 Descriptors: polluted-soils bioremediationAbstract: Biological remediation of contaminated soil is an effective method of reducing risk to human and ecosystem health. Bacteria and plants might be used to enhance remediation of soil pollutants in situ. This study assessed the potential of bacteria (12 isolates), plants (16 forage grasses), and plant-bacteria associations (selected pairings) to remediate 2-chlorobenzoic acid (2CBA)-contaminated soil. Initially, grass viability was assessed in 2CBA-contaminated soil. Soil was contaminated wi th 2CBA, forage grasses were grown under growth chamber conditions for 42 or 60 d, and the 2CBA concentration in soil was determined by gas chromatography. Only five of 16 forage grasses grew in 2CBA-treated (816 mg/kg) soil. Growth of Bromus inermis had no effect on 2CBA concentration, whereas Agropyron intermedium, B. biebersteinii, A. riparum, and Elymus dauricus decreased 2CBA relative to nonplanted control soil by 32 to 42%. The 12 bacteria isolates were screened for their ability to promote the germ ination of the five grasses in 2CBA-contaminated soil. Inoculation of A. riparum with Pseudomonas aeruginos

Phytoremediation : 植物による環境/土壌浄化

The low impact development (LID) approach has been recommended as an alternative to traditional stormwater design. Research on individual LID practices such as bioretention, pervious pavements, and grassed swales has increased in recent years. Bioretention cells have been effective in retaining large volumes of runoff and pollutants on site, and consistently reduced concentrations of certain pollutants such as metals. However, retention of certain pollutants such as nitrate–nitrogen and phosphorus has been problematic. Porous pavements have been extremely effective in infiltrating stormwater runoff. Concerns have been raised about groundwater contamination, but research has shown that this is not a problem in most settings. Green roofs have been found to retain a large percentage of rainfall (63% on average) in a variety of climates. A common thread across bioretention, green roofs and grassed swales was found: the export of phosphorus. The issue appears to be linked to high phosphorus levels in the soil media, or possibly to fertilization of turf or planted areas. Solutions to this problem have been recommended. Contrary to popular belief, research has shown that bioretention and pervious pavements continue to infiltrate even with frost in the ground. Although issues have been identified with retention of certain pollutants, the LID approach has been found to result in increased retention of stormwater and pollutants on site, mimicking pre-development hydrologic function. Future research needs have also been identified.

https://clear.uconn.edu/projects/tmdl/library/papers/dietz_2007.pdf

Low Impact Development Practices: A Review of Current Research and Recommendations for Future Directions

Nutrient and sediment removal by stormwater biofilters: A large-scale design optimisation study

Storm-water pollutants are widely recognized as a major cause of surface water quality degradation. Most storm-water treatment efforts have focused on capture of particles and particle-associated pollutants, but oftentimes half or more of pollutant loads can be attributed to the dissolved phase. Dissolved pollutants are more mobile, bioavailable, and are captured via different mechanisms than particles. Low-impact development storm-water control measures such as bioretention are being used to infiltrate storm water to reduce storm-water volume as well as to capture storm-water pollutants. Bioretention systems have proven effective at capturing both dissolved and particulate storm-water pollutants. Herein the authors present a state-of-the-art review of dissolved storm-water pollutant sources and typical concentrations, removal mechanisms, and fate in bioretention cells covering three pollutant classes: (1) nutrients (i.e., phosphorus and nitrogen), (2) toxic metals, and (3) organic compounds, incl...

Review of dissolved pollutants in urban storm water and their removal and fate in bioretention cells

Snowmelt pollutant removal in bioretention areas

A bioretention media is a stormwater treatment option designed to reduce peak runoff volumes and improve water quality through soil infiltration and plant mitigation. To investigate the heavy metal removal in a bioretention media in a cold climate setting, a small pilot sized bioretention box was built in Trondheim, Norway. The system was sized using the Prince Georges County bioretention design method from 1993. Three runoff events, created using historical data, were undertaken in April 2005 and then again in August 2005. Both the peak flow reduction and the total volume reduction were significantly lower in April compared to August. Peak flow reduction was 13% in April versus 26% in August and the total volume reduction was 13% in April versus 25% in August. Metal retention was good for both seasons with 90% mass reduction of zinc, 82% mass reduction of lead and 72% mass reduction of copper. Plant uptake of metals was documented between 2 to 7%; however adsorption and mechanical filtration through the mulch and soil column were the most dominant metal retention processes. The metal retention was independent of the selected hydraulic loading rates (equivalent to 1.4–7.5 mm h−1 precipitation) showing that variable inflow rates during this set of events did not affect the treatment efficiency of the system.

Heavy Metal Removal in Cold Climate Bioretention

This paper evaluates the performance and winter hydrology of two small-scale rain gardens in a cold climate coastal area in Trondheim, Norway. One rain garden received runoff from a small residential watershed over a 20 month study period while the second rain garden with a shorter study period of 7 months was used as a control. The objective of the study was to investigate the extent to which cold climatic conditions would influence the hydrology and performance of the rain gardens. The hydraulic detention, storm lag time and peak flow reduction were measured and compared seasonally. No significant difference between seasonal lag time could be found, but there was a clear decreasing trend in lag time between rain, rain-on-snow and snowmelt. The average peak flow reduction for 44 storms in the study period was 42% compared to 27% for the winter seasons, indicating that the performance of the rain garden is reduced in the cold season (below 0 °C). The average hydraulic detention time for the rain garden was 0·84 ( ± 0·73) with runoff inflow and 1·91 ( ± 3·1) with only precipitation. A strong positive correlation was found between the time since the last wetting event and lag time, and between air temperature and hydraulic detention. This indicates that the time between events and seasonal air temperatures are key parameters in the hydraulic performance of cold climate rain gardens. The rain gardens were not used for snow storage areas, and a volume requirement for this was not evaluated in the study. Copyright © 2007 John Wiley & Sons, Ltd.

Sveinn T. Thorolfsson

Papers

Snowmelt pollutant removal in bioretention areas

Heavy Metal Removal in Cold Climate Bioretention

Seasonal climatic effects on the hydrology of a rain garden

Regional frequency analysis of extreme precipitation with consideration of uncertainties to update IDF curves for the city of Trondheim

An evaluation of applying existing bioretention sizing methods to cold climates with snow storage conditions.