Recent progress in consolidated bioprocessing

https://www.tpbin.com/Uploads/Subjects/b48cb714-739a-44a7-ad96-29503eac42f8.pdf

Improved lignocellulose conversion to biofuels with thermophilic bacteria and thermostable enzymes.

We present here a broad overview of the field of metabolic engineering, describing in the first section the key fundamental principles that define and distinguish it, as well as the technological and intellectual developments over the past approximately 20 years that have led to the current state of the art. Discussion of concepts such as metabolic flux analysis, metabolic control analysis, and rational and combinatorial methods is facilitated by illustrative examples of their application drawn from the extensive metabolic engineering literature. In the second section, we present some of the rapidly emerging technologies that we think will play pivotal roles in the continued growth of the field, from improving production metrics to expanding the range of attainable compounds.

Metabolic Engineering: Past and Future

Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process

/pdf/advances-in-consolidated-bioprocessing-systems-for-10xtqwl71h.pdf

T. Atkinson

Papers

Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production.