Showing papers by "T.S. Chandra published in 2020"

Studies on expression levels of pil Q and fli P genes during bio-electrogenic process in Kluyvera georgiana MCC 3673

Abstract: The bacterium Kluyvera georgiana MCC 3673 transfers electrons directly to the electrode for bio-electricity generation in microbial fuel cell (MFC). This could be due to the formation of biofilm on the surface of electrode or with through the extracellular appendages, or both. The role of extracellular appendages pili and flagella in exo-electron transfer mechanism was investigated. The expression level of the genes fli P and pil Q for pili and flagella, respectively, in K. georgiana MCC 3673 was compared in MFC and in shake flask. The transcript analysis was done by qRT-PCR at different times and conditions. The expression level of pil Q transcript in K. georgiana MCC 3673 showed over twofold higher expression during bio-electrogenic process, compared to the one inoculated in shake flask. Similarly, fli P had also showed similar kind of expression in MFC compared to that in shake flask. Higher level of pil Q and fli P transcripts were observed throughout bio-electrogenic process. The level of pil Q was found to be nearly fourfold higher in biofilm-forming cells forming compared to the cells in suspension form. The obtained results suggest that flagella have a role in movement of bacterium towards electrode for donating the electron in absence of oxygen, and pili aiding in adhering on the surface of electrode and forming biofilm. The cumulative effect of fli P and pil Q resulted in exo-electron transfer to the electrode and bio-electricity generation process. The open circuit potential (OCV) of + 0.7 V was produced with the maximum power density of 393 ± 14 mW/m2 in MFC.

2D MoS2 for sweat based biosensor application

Abstract: The MoS2 2D nanosheets prepared by the hydrothermal method are characterized for its structural and morphological features. The structural studies show the pure form of MoS2 with hexagonal phase. The morphological study done with Tunnelling Electron Microscope shows that there are 6 to 10 2D atomic layers of MoS2. The sensitivity of this 2D MoS2towards Sodium ion was done using Cyclic Voltammetry. The results confirm that 2D MoS2 modified Carbon electrodes are good candidates for selective sensing of sodium ion in sweat. The sensing mechanism is explained.