基礎講座 電気泳動(Electrophoresis)

Mechanistic analysis of solute transport in an in vitro physiological two‐phase dissolution apparatus

High-Performance Thin-Layer Chromatography (HPTLC)

Thin-layer chromatography: challenges and opportunities

Computerized design of separation strategies by reversed-phase liquid chromatography: development of DryLab software

Modification of a horizontal sandwich chamber for thin-layer chromatography

Pressurized planar electrochromatography (PPEC) is the mode which offers much higher separation efficiency in comparison to conventional planar chromatography, including both higher performance and much higher speed of separation In this paper, we present a new device for performing PPEC in which the whole area of the chromatographic plate is pressurized Both electrodes (anode and cathode) are washed with the mobile phase during the experiment, which prevents gas bubbles from collection in the region of the electrodes This device enables directly controlling the flow rate of the mobile phase during the electrochromatography process Mobile phase control offers the possibility of researching the influence of various properties of the PPEC system on separation efficiency One important relationship to investigate is plate height vs mobile phase flow rate This relationship helps to choose the optimal value of the mobile phase flow rate during the separation process Considerable difference in shape of this relationship is demonstrated for conventional planar chromatography plates and high performance planar chromatography plates Examples of the influence of some properties of the separating system on flow rate of the mobile phase are demonstrated, such as the buffer concentration in the mobile phase, the pH value of the buffer solution of the mobile phase, the type of chromatographic plate, and the voltage applied to the electrodes

Apparatus for pressurized planar electrochromatography in a completely closed system

Separation of coumarins from Archangelica officinalis in high-performance liquid chromatography and thin-layer chromatography systems.

A horizontal DS chamber for TLC has been adapted for planar electrochromatography in closed system. A test dye mixture was separated on pre-wetted RP-8 and RP-18 chromatographic plates with acetonitrile-buffer as mobile phase. A potential of 2 kV was applied to a 10-cm plate to create the electric field. Two modes of electrochromatogram development were applied — in open and closed (pressurized) systems. The disadvantages of the open system — evaporation of the mobile phase from the plate and excessive flow of mobile phase to the surface of the adsorbent layer during development — were eliminated by use of the closed system. Highly reproducible retention was achieved during planar electrochromatography in the closed system when the adsorbent layer of the plate was pre-wetted and equilibrated with a solution of the mobile phase. Examples are presented of electrochromatograms obtained from the test dye mixture under different operating conditions. Separation efficiency in planar electrochromatography was hi...

Adaptation of a horizontal DS chamber to planar electrochromatography in a closed system

Influence of sample application mode on performance of pressurized planar electrochromatography in completely closed system.

Tadeusz H. Dzido

Papers

Modification of a horizontal sandwich chamber for thin-layer chromatography

Apparatus for pressurized planar electrochromatography in a completely closed system

Separation of coumarins from Archangelica officinalis in high-performance liquid chromatography and thin-layer chromatography systems.

Adaptation of a horizontal DS chamber to planar electrochromatography in a closed system

Influence of sample application mode on performance of pressurized planar electrochromatography in completely closed system.