PROBLEM TO BE SOLVED: To provide a method for preparing a biosample, capable of effectively extracting and solubilizing protoporphyrins from a sample to be inspected to efficiently measure the same, without having to affect a detection system.SOLUTION: The biosample is prepared by adding a solubilizing agent, such as, a surfactant or the like to the sample to be inspected to solubilize protoporphyrins, and the cancer of digestive system is detected by detecting protoporphyrins by using the biosample.

Method for preparing biosample

PROBLEM TO BE SOLVED: To provide a method and device capable of noninvasively diagnosing a tumor of the digestive system. SOLUTION: The detection method and device of protoporphyrins in a biological sample comprise an immunoassay for detecting protoporphyrins in a biological sample using an antibody to one compound selected from the group consisting of protoporphyrins. The antibody is fixed at a prescribed position of a membrane carrier to form an uptake site, and protoporphyrins in the biological sample can be detected by an immunochromatography for competitively binding them and labeled protoporphyrins to the uptake site. COPYRIGHT: (C)2008,JPO&INPIT

Takeuchi Rikiya

Papers

Immunological detection method and device of protoporphyrins in biological sample