Tawakol A. El-Shourbagy

TL;DR: The most recent advances in sample preparation, separation, and the mass spectrometric aspects of high-throughput quantitative bioanalysis of drug and metabolites in biological matrices are reviewed.

TL;DR: A new sample preparation technique, salting-out assisted liquid-liquid extraction with acetonitrile, for high-throughput good laboratory practice sample analysis using LCMS, which indicates that the method is rapid, reliable and suitable for regulated bioanalysis.

TL;DR: The assay has proven rugged and specific and has been employed to generate data in support of preclinical studies and could be used for the development of bioanalytical assays to provide preclinical and clinical support for other protein drug candidates and, furthermore, for the validation of biomarkers discovered from proteomic research.

TL;DR: The use of a mass spectrometry friendly organic salt, ammonium acetate, is reported for the first time, as a salting-out reagent in SALLE with acetonitrile for the simultaneous quantitation of an Abbott investigational new drug ABT-869 and its hydrophilic metabolite in human plasma.

TL;DR: A high-throughput salting-out assisted liquid/liquid extraction (SALLE) method with acetonitrile and mass spectrometry compatible salts for simultaneous LC-MS/MS analysis of SS and SSA was used for a bioequivalence study.