Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

System and method including analytical units

A polymer comprising repeat units of the formula: wherein R1 is independently in each occurrence C1-20 hydrocarbyl or C1-20 hydrocarbyl containing one or more heteroatoms of S, N, O, P or Si or both R1 may form, with the 9-carbon of the fluorene ring, a C5-20 ring structure containing one or more heteroatoms of S, N, or O; R2 is independently in each occurrence C1-20 hydrocarbyl, C1-20 hydrocarbyloxy, C1-20 thioether, C1-20 hydrocarbyloxycarbonyl, hydrocarbylcarbonyloxy, or cyano; a is independently in each occurrence a number from about 0 to about 1; m is a number from about 1 to about 2; and n is a number from about 3 or greater.

Fluorene-containing polymers and compounds useful in the preparation thereof

The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.

Isolation of nucleic acid

This invention relates to magnetic nano sized particles having a glass surface. This invention also relates to methods for making them, as well as to suspensions thereof and their uses for the purification of DNA or RNA in particular in automated processes.

Magnetic glass particles, method for their preparation and uses thereof

This invention provides biochip cartridges and instrument devices for the detection and/or analysis of target analytes from patient samples.

Integrated multiplex target analysis

Magnetic glass particles are prepared containing a magnetic core coated with a glass layer having a substantially pore-free glass surface. The particles are used for separating biological material such as nucleic acids. A preferred process of preparing the particles is by forming a mixture of magnetic cores with a sol formed from an alcohol and a metal alkoxide, spray-drying the mixture to coat the cores with a layer of gelled sol, and heating the coated cores to obtain the magnetic glass particles. Preferably, the particles have an average particle size of less than 100 μm and any pores of the glass surface have a diameter of less than 10 nm. The magnetic core may be a composite material containing a mica core and magnetite particles immobilized on the mica core, and the glass layer may contain boron oxide. Magnetic core materials include magnetite (Fe3O4) and Fe2O3. In using the magnetic glass particles to separate a biological material, the particles are contacted with a fluid containing the biological material such that the biological material binds to the glass surface, and the bound biological material is separated from the fluid such as by using a magnetic field. Before applying a magnetic field, the magnetic particles may sediment when contacted with the biological material.

Particles having a magnetic core and outer glass layer for separating biological material

The present invention provides a method for an enzyme reaction for nucleic acid and compound for segregating nucleic acid, and relates to a method for segregating nucleic acid, including that nucleic acid sample having nature shape in fluid contacts with magnetic particles with glass surface when nucleic acid may be connected to surface of glass, said glass basically has not holes or has holes with diameter less than 10nm, and nucleic segregated from fluid.

Enzyme reaction method for nucleic acid and composition for separating nucleic acid

Magnetic particle (A) with an external glass surface that is non-porous or has pores with a dia. <10 nm. Also claimed is a ferromagnetic particle (B) with a glass surface.

Magnetic particles with, opt. porous, glass surface

The present invention provides an attachment or extension for pipettes and pipette tips. The attachment comprises separation materials which are preferably suitable for isolating nucleic acids from liquids. The separation materials are present in the form of a filter disc in a holder which only slightly increases the pipette volume and thus generates little dead volume.

Pipette tip with separation material

The device has a cylindrical wall (200) forming an opening in a section (A), where the opening is in fluidic connection with an outlet opening of a pipette. A disk-like filter (600) is arranged transverse to a longitudinal axis (L) in the section. A side lying opposite to the opening is limited by a wall (210). Volume (300) in the section between the filter and another opening of another section (B) amounts to range of 0.01 percentage to 5 percentage of maximum transfer volumes of a pipette tip (C). Dimension of the latter opening is measured in a range of 0.5 mm to 2 mm. An independent claim is also included for a method for manufacturing nucleic acids.

Thomas Walter

Papers

Particles having a magnetic core and outer glass layer for separating biological material

Enzyme reaction method for nucleic acid and composition for separating nucleic acid

Magnetic particles with, opt. porous, glass surface

Pipette tip with separation material

Pipette tip with separating material