Timothy K. Lu

TL;DR: This work demonstrates the feasibility and benefits of using engineered enzymatic bacteriophage to reduce bacterial biofilms and the applicability of synthetic biology to an important medical and industrial problem.

TL;DR: Two complementary synthetic genetic counters in Escherichia coli can count up to three induction events: the first, a riboregulated transcriptional cascade, and the second, a recombinase-based cascade of memory units that permit counting of varied user-defined inputs over a range of frequencies and can be expanded to count higher numbers.

TL;DR: This work uses CRISPR-Cas technology to create antimicrobials whose spectrum of activity is chosen by design, and shows that RGNs enable modulation of complex bacterial populations by selective knockdown of targeted strains based on genetic signatures.

TL;DR: This work describes a strategy for efficiently assembling synthetic genetic circuits that use recombinases to implement Boolean logic functions with stable DNA-encoded memory of events and created two-bit digital-to-analog converters, which should be useful in biotechnology applications for encoding multiple stable gene expression outputs using transient inputs of inducers.

TL;DR: It is shown that suppressing the SOS network in Escherichia coli with engineered bacteriophage enhances killing by quinolones by several orders of magnitude in vitro and significantly increases survival of infected mice in vivo.