Certain diets cause a marked reduction in milk fat production in ruminants. Commonly referred to as milk fat depression (MFD), the mechanism involves an interrelationship between rumen microbial processes and tissue metabolism. Numerous theories to explain this interrelationship have been proposed and investigations offer little support for theories that are based on a limitation in the supply of lipogenic precursors. Rather, the basis involves alterations in rumen biohydrogenation of dietary polyunsaturated fatty acids and a specific inhibition of mammary synthesis of milk fat. The biohydrogenation theory proposes that under certain dietary conditions, typical pathways of rumen biohydrogenation are altered to produce unique fatty acid intermediates that inhibit milk fat synthesis. Trans-10, cis-12 conjugated linoleic acid (CLA) has been identified as one example that is correlated with the reduction in milk fat. Investigations with pure isomers have shown that trans-10, cis-12 CLA is a potent inhibitor of milk fat synthesis, and similar to diet-induced MFD, the mechanism involves a coordinated reduction in mRNA abundance for key enzymes involved in the biochemical pathways of fat synthesis. A more complete identification of these naturally produced inhibitors of fat synthesis and delineation of cellular mechanisms may offer broader opportunities for application and understanding of the regulation of lipid metabolism.

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Nutritional regulation of milk fat synthesis.

There has been great interest in dry matter intake regulation in lactating dairy cattle to enhance performance and improve animal health and welfare. Predicting voluntary dry matter intake (VDMI) is complex and influenced by numerous factors relating to the diet, management, housing, environment and the animal. The objective of this review is to identify and discuss important metabolic factors involved in the regulation of VDMI and their integration with metabolism. We have described the adaptations of intake and metabolism and discussed mechanisms of intake regulation. Furthermore we have reviewed selected metabolic signals involved in intake regulation. A substantial dip in VDMI is initiated in late pregnancy and continues into early lactation. This dip has traditionally been interpreted as caused by physical constraints, but this role is most likely overemphasized. The dip in intake coincides with changes in reproductive status, fat mass, and metabolic changes in support of lactation, and we have described metabolic signals that may play an equally important role in intake regulation. These signals include nutrients, metabolites, reproductive hormones, stress hormones, leptin, insulin, gut peptides, cytokines, and neuropeptides such as neuropeptide Y, galanin, and corticotrophin-releasing factor. The involvement of these signals in the periparturient dip in intake is discussed, and evidence supporting the integration of the regulation of intake and metabolism is presented. Still, much research is needed to clarify the complex regulation of VDMI in lactating

SYMPOSIUM: DRY MATTER INTAKE OF LACTATING DAIRY CATTLE Integration of Metabolism and Intake Regulation: A Review Focusing on Periparturient Animals

Integration of metabolism and intake regulation: a review focusing on periparturient animals.

Altering Milk Composition by Feeding

Protein-Energy Interrelationships in Dairy Cows

Eighty Friesian heifers were used to study the effect on milk production of two fixed levels of intake at two stages of the lactation. The rations were estimated to contain 7·9 and 6·2 kg starch equivalent per day, with adequate protein content. The heifers were allocated at random to these two levels of intake for weeks 1·9 of lactation and re-allocated at random to them for weeks 10–18 of lactation. Apart from minor variation the heifers were all fed alike for the remainder of the lactation.The higher level of feeding increased yields of milk and milk solids and solids-not-fat content in each period but had a smaller effect in mid- than in early lactation, a trend which was shown to continue further into later lactation. It also conserved body reserves.There was a residual effect in weeks 10–18 from the level of feeding in weeks 1–9 of lactation. This was directly additive to the effects of the current level of feeding. Those animals which had received the higher level of feeding in weeks 1–9 yielded more milk of higher solids-not-fat content than those which had had the lower level of feeding in weeks 1–9. The former group gained less live weight. The effect on milk production over the full lactation from additional feeding in early lactation was three times that observed in early lactation itself. Additional feeding in mid-lactation did not have a residual effect.The absolute output of milk per unit of food over the 18-week period was greatest for that group which had received the lower level of feeding throughout, followed by the group which received the higher level of feeding in weeks 1–9 only. This superiority was achieved at the expense of body reserves. There was a negative regression of live-weight change on milk production.Estimated intakes of metabolizable energy agreed closely with requirements for milk production as indicated by the Agricultural Research Council (1965).

Experiments on the nutrition of the dairy heifer: VIII. Effect on milk production of level of feeding at two stages of the lactation

Reviews of the progress of dairy science: long term effects of plane of nutrition on the performance of the dairy cow.

Food utilization by lactating dairy cows was studied in a randomized experiment covering the first 24 weeks of lactation, with further observations on performance over the remainder of the lactation under farm conditions. Over a 3-year period 90 firstcalf cows each took part in the experiment for one lactation, making six groups of 14–16 animals.For the experimental period a fixed basal diet was given of hay, concentrates, and wet brewer's grains. One group received only the basal ration. Four groups received in addition a fixed concentrates supplement for 4, 8, 12, 16 weeks from calving onwards respectively. The sixth group also had supplementary concentrates for 16 weeks but for weeks 5–12 inclusive the amount was doubled so that this group's diet simulated broadly ad libitum feeding in early lactation.Milk yield was increased by extra concentrates. The greater part of the effect was apparent in the first 2 weeks of different levels of intake, with a further build-up over the next 6–8 weeks. The size of the response fell as time from calving to initiation of different intakes increased. Additional food increased and delayed peak yield level. Persistency of milk yield was not affected by fixed differences in intake. Rate of liveweight change was improved by extra concentrates, less so in the third compared with the first month on treatment.A higher level of feeding in early lactation led to a greater partition of nutrients in mid-lactation to milk at the expense of gains in body reserves. This advantage to milk production extended to the end of lactation. In it the greatest response per unit concentrates was obtained from supplementation in the first 4 weeks of lactation but the total effect was increased by giving a double allowance of concentrates, and by extending the period of extra feeding to 8 weeks after calving.Solids-not-fat content and yield, and fat yield but not fat content, showed a similar response pattern to milk yield.Animal-to-animal variation in output on constant diets was characterized by negative regressions of live-weight change on milk yield. The regression coefficients were greater in early than in mid-lactation but they were constant in size at any one time for the various groups. A change in ration induced a change in output of the individual in which that production pathway favoured naturally by the animal was the more affected.

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S002185960007204X

Experiments on the nutrition of the dairy heifer:IX. Food utilization in lactation

Mean digestible energy (DE) intakes of 147 cows of three parities receiving three levels of DE including one ad libitum (about 2·2, 2·6 and 3·3 multiples of maintenance (MM)) drawn from three mixed diets containing hay and 60, 75 or 90% compound were calculated.Rumen samples were taken from three-quarters of the cows at monthly intervals throughout the experiment. Molar proportions of volatile fatty acids (VFA) in the rumen were not affected by stage of lactation over the 36 weeks of the experiment. The proportion of acetic acid decreased and that of propionic acid increased with greater intakes and with higher proportions of compound in the diet. The proportion of n-butyric acid was little affected by level of intake but decreased with increasing proportions of compound. In young, non-lactating cattle given the same diets but at lower levels of intake, VFA proportions were unaffected by diet composition at intakes of about 0·7 MM but at intakes of about 1·3 MM the proportion of acetate decreased and that of propionate increased when the proportion of compound was increased from 75 to 90%.The efficiency of milk energy production in relation to DE or metabolizable energy (ME) above maintenance decreased with increasing level of intake but was little affected by the proportion of compound. Partition towards live weight increased with level of intake in early lactation but not in late lactation. It also increased with higher proportions of compound in mid and late lactation but not in early lactation.Estimates of the ME requirement for live-weight change (LWC) were in reasonable agreement with recently published standards in early lactation when live weight was decreasing, but later in lactation when live-weight gain was occurring, a much higher value was calculated which is difficult to reconcile with these standards.Rumen VFA proportions were related to dietary fibre concentration and level of intake additively. The relationship to VFA proportions was close for milk fat concentration, but less so for energy partition towards live weight and none was apparent for the efficiency of ME utilization for milk energy production. It is suggested that both the reduction in milk fat concentration and the increase in milk yield in response to reductions in the fibre content of diets may be independently related to the increase in the proportion of propionate in the rumen VFA.It is concluded that further progress in studies of the dietary factors affecting the efficiency of milk production will require measurements of nutrient uptake from the digestive tract and description of milk production and LWC in terms of their chemical composition rather than energy alone.

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0021859600081284

Influence of plane of nutrition and diet composition on rumen fermentation and energy utilization by dairy cows

Summary Seventy-six first-calf cows (heifers), 32 second·calf cows and 39 adult cows participated in a trial in which three diets composed of 60:40, 75:25, 90:10 proportions of compound to hay (hay dry-matter digestibility 0·6) were given for the first 36 weeks of lactation in amounts to provide daily an estimated 125, 156 MJ and ad libitum digestible energy (DE) for heifers, and 156, 187 MJ and ad libitum DE for the other parities. The ad libitum intakes were 150 and 200 MJ DE daily for heifers and for older cows respectively. Greater compound content of the diet and larger daily allowance of DE were associated with increased occurrence of mastitis and lameness but not digestive upsets. Dry·matter (D.M.) digestibility was increased with a greater proportion of compound in the diet but was not affected by the amount consumed or stage of lactation. Milk yield and live·weight change were increased linearly and additively by a greater intake of digestible dry matter and by a greater proportion of compound in the diet, throughout the lactation save for no effect of diet composition on live·weight change in early lactation. On equal feed intakes older cows gave more milk and lost more live weight than heifers in early lactation but not thereafter; responses to additional feed were equal for all parities studied. Milk fat concentration was reduced both by increased allowance and by the high compound diet. Milk solids·not·fat (SNF) concentration was not affected by the treatments. Yield of milk fat was not affected by amount eaten and was reduced by the 90:10 diet. Yields of SNF and milk energy were increased by amount eaten and by increased compound in the diet. Thirty-two primiparous cows continued on their experimental treatments without interruption to the end of the second lactation. In the second lactation the effects on yields of milk, milk energy, and SNF were magnified indicating a cumulative effect from lactation to lactation. Little cumulative effect was observed on either milk fat yield, or concentrations of fat and SNF in milk. More generous feeding was associated with reduced milk output/unit D.M. consumed in the first but not the second lactation. More generous feeding improved live·weight change less in the second than in the first lactation. No cumulative effects over two lactations were observed consequent upon variation in diet composition.

Valerie J. Broster

Papers

Experiments on the nutrition of the dairy heifer: VIII. Effect on milk production of level of feeding at two stages of the lactation

Reviews of the progress of dairy science: long term effects of plane of nutrition on the performance of the dairy cow.

Experiments on the nutrition of the dairy heifer:IX. Food utilization in lactation

Influence of plane of nutrition and diet composition on rumen fermentation and energy utilization by dairy cows

The influence of plane of nutrition and diet composition on the performance of dairy cows