A biological control agent comprising the mycoparasite Trichoderma harzianum T-315 (ATCC No. 20671), which is characterized by fungicidal activity against fungi of the genera Pythium, Rhizoctonia, Sclerotium and Fusarium. This strain is useful for controlling damping-off, root-rot, crown-rot and neck-rot in seedling crops. This strain is also resistant to chemical pesticides and soil-sterilants and useful for integrated biological and chemical control of soil-borne pathogens.

Novel isolate of trichoderma, fungicidal compositions containing said isolate and use thereof

Microbial enzyme preparations having fungicidal activity comprising an enzyme preparation capable of degrading the cell wall or parts of the cell wall of fungal mycelium, fungal spores or other vital parts of a fungal structure, compositions comprising said preparations, and synergistic preparations further comprising a conventional fungicide.

Fungicidally active preparations

A process for improving the filterability of wort or beer comprising treating said wort or beer with an enzymatic product produced by Disporotrichum dimorphosporm exhibiting xylanase activity in an amount effective to improve the filterability.

Production of beer

The present invention provides processes for production of wort and beer wherein the mash liquefaction comprises jet cooking and application of microbial mashing enzymes.

Beer mashing process

Mutanase (α-(1→3)-glucanase) is a little-known inductive enzyme that is potentially useful in dentistry. Here, it was shown that the cell wall preparation (CWP) obtained from the fruiting body or vegetative mycelium of polypore fungus Laetiporus sulphureus is rich in α-(1→3)-glucan and can be successfully used for mutanase induction in Trichoderma harzianum. The content of this biopolymer in the CWP depended on the age of fruiting bodies and increased along with their maturation. In the case of CWP prepared from vegetative mycelia, the amount of α-(1→3)-glucan depended on the mycelium age and also on the kind of medium used for its cultivation. All CWPs prepared from the individually harvested fruiting body specimens induced high mutanase activity (0.53-0.82 U/mL) in T. harzianum after 3 days of cultivation. As for the CWPs obtained from the hyphal mycelia of L. sulpureus, the maximal enzyme productivity (0.34 U/mL after 3 days of incubation) was recorded for CWP prepared from the 3 week-old mycelium cultivated in Sabouraud medium. Statistically, a high positive correlation was found between the total percentage content of α-(1→3)-glucan in the CWP and the mutanase activity.

Comparative Studies on the Induction of Trichoderma harzianum Mutanase by α-(1→3)-Glucan-Rich Fruiting Bodies and Mycelia of Laetiporus sulphureus

The filterability of wine and must obtained from grapes infected with the mold Botrytis cinerea is improved by treatment thereof with an enzyme product produced by cultivating the microorganism Trichoderma harzianum The enzyme product exhibits both alpha- and beta-glucanase activity

Villettaz Jean-Claude

Papers

Enzymatic treatment of wine and must

Verfahren zur verbesserung der filtrierfaehigkeit von presssaft und wein aus mit botrytis infizierten trauben.

Procede pour ameliorer la filtrabilite des mouts de raisins infectes par botrytis et des vins produits par fermentation de ces mouts