Showing papers by "Vladimir A. Izumrudov published in 2018"
TL;DR: A review of the results of studies on the synthesis of polyelectrolyte coatings on charged surfaces can be found in this paper, where the authors discuss the potential of using the layer-by-layer (LbL) method to fabricate nanofilms.
Abstract: The review concerns the results of studies on the synthesis of polyelectrolyte coatings on charged surfaces. These coatings represent nanostructured systems with clearly defined tendency to self-assembly and self-adjustment, which is of particular interest for materials science, biomedicine and pharmacology. A breakthrough in this area of knowledge is due to the development and introduction of a new technique, so-called layer-by-layer (LbL) deposition of nanofilms. The technique is very simple, viz., multilayers are formed as a result of alternating treatment of a charged substrate of arbitrary shape with water-salt solutions of differently charged polyelectrolytes. Nevertheless, efficient use of the LbL method to fabricate nanofilms requires meeting certain conditions and limitations that were revealed in the course of research on model systems. Prospects for applications of polyelectrolyte layers in various fields are discussed.The bibliography includes 58 references.
23 citations
TL;DR: Polyanions are found to enhance the proteolytic degradation of GAPDH by proteinase K and thermolysin and can be useful for treatment of pathologies associated with amyloid aggregation.
Abstract: The use of polyelectrolytes is a prospective approach to form nanocomplexes to transport different compounds including proteins. In many cases, the bound protein should be digested after delivery to the target. In the present work, we studied proteolysis of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the complexes with polyelectrolytes. We have found polyanions to enhance the proteolytic degradation of GAPDH by proteinase K and thermolysin. This effect seems to be caused by destabilization of the protein structure. However, this destabilization is reversible since the release of the enzyme from the complexes with polymers (even tightly bound with the protein such as sulfated polymers and supercharged pyridinium polycations) was accompanied by partial or complete reactivation of GAPDH, depending on the polymers and conditions. Finally, we observed that complexation with sulfated polymers enhances the proteolytic degradation of prion fibrils by proteinase K. The obtained results can be useful for treatment of pathologies associated with amyloid aggregation.
13 citations
TL;DR: In this article, a quaternization of the parent poly(4-vinylpyridine) with different alkylating agents, providing the possibility to perform the modification as a one-step reaction, which occurs in mild conditions with a controllable degree of conversion.
Abstract: Quite recently, we reported the synthesis of supercharged polycations bearing pH-insensitive double-charged repeat units with either three or five methylene groups in the space between charges. The developed approach is based on the quaternization of the parent poly(4-vinylpyridine) with different alkylating agents, providing the possibility to perform the modification as a one-step reaction, which occurs in mild conditions with a controllable degree of conversion. In the present work, we used the above approach for preparing and investigating supercharged polyplexes (polyelectrolyte complexes of nucleic acids), in particular to elucidate the reason for the key feature, i.e., the clearly defined stability of the polyplexes formed by supercharged polyamines. The main findings of the experimental study were confirmed by the results of full atomic modeling, and the principal regularities responsible for the structure, stability, and properties of the supercharged polyplexes have been elucidated for the first...
11 citations