Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems

The lipases obtained from the genus Aspergillus present remarkable importance in biotechnological applications, and numerous studies have reported the importance of the fermentation parameters, such as nutrients, temperature and fermentation time. Moreover, many Aspergillus spp. lipases present several properties of immense industrial importance, such as their pH and temperature stability and excellent enantioselectivity. Different strategies have been used in order to immobilize crude or purified Aspergillus spp. lipases. Hence, Aspergillus spp. lipases have been studied for different industrial applications such as in the food and detergent industries, and also in the kinetic resolution of pharmaceuticals and chiral intermediates. This review highlights the production, purification, characterization, applications and immobilization of lipases from Aspergillus spp.

Aspergillus sp. lipase: Potential biocatalyst for industrial use

Publisher Summary This chapter discusses the physiology and biotechnology of Aspergillus species. The genus Aspergillus is found worldwide and consists of more than 180 officially recognized species. Molecular and genetic studies of Aspergillus species tend to concentrate on four thoroughly investigated species: A. nidulans, A. niger, A. oryzae, and A. fumigatus. The physiology of the Aspergillus such as life cycle, physiological responses and signal transductions, and carbon and nitrogen metabolisms are presented in this chapter. Most Aspergillus species, except A. fumigatus, have both sexual and asexual processes for spore duplication. A few, including A. nidulans, are also able to reproduce via a parasexual cycle. The capacities of microbial species to survive and respond physiologically to changes in their environment enable these species to exist under a broad range of conditions. Several signaling pathways are involved in Aspergillus cell growth and differentiation, and indicate links between cell developments to metabolite biosynthesis. The molecular biology of Aspergillus is also explained with the help of its gene expression, and by discussing the molecular biology approach of production of protein by Aspergillus species. The chapter presents the industrial application of the Aspergillus species such as citric acid production, production of extracellular enzymes, role of Aspergillus in the process of biotransformation, production of secondary metabolites, environmental applications, and production of other industrial products.

Physiology and Biotechnology of Aspergillus

Purification and characterization of acidic lipase from Aspergillus niger NCIM 1207.

Sugar esters are non-ionic surfactants that can be synthesized in a single enzymatic reaction step using lipases. The stability and efficiency of lipases under unusual conditions and using non-conventional media can be significantly improved through immobilization and protein engineering. Also, the development of de novo enzymes has seen a significant increase lately under the scope of the new field of synthetic biology. Depending on the esterification degree and the nature of fatty acid and/or sugar, a range of sugar esters can be synthesized. Due to their surface activity and emulsifying capacity, sugar esters are promising for applications in food industry.

W.S Davidson

Papers

Purification and characterization of an alkaline thermostable lipase from Aspergillus carneus