The invention relates to an ultrafine-grinding assisted enzymatic-hydrolysis based preparation method of wheat bran oligosaccharides. The wheat bran oligosaccharides are prepared by adopting the ultrafine grinding technology and the composite enzymatic hydrolysis technology by mainly taking wheat bran as a raw material. The ultrafine-grinding assisted enzymatic-hydrolysis based preparation method of the wheat bran oligosaccharides comprises the following steps: degrading starch in the wheat bran by using a high-temperature resistant alpha-amylase and glucoamylase, and degrading proteins in the wheat bran by using alkaline proteases; then hydrolyzing celluloses and hemicelluloses in the wheat bran by using cellulases and xylanases; and concentrating a supernatant by enzymatic hydrolysis, and drying to obtain the wheat bran oligosaccharide. The wheat bran is treated by adopting the ultrafine grinding technology so that the cell wall structure is destroyed, starch and proteins in the wheat bran are degraded by adopting a complex enzyme system, and celluloses and hemicelluloses in the wheat bran are hydrolyzed by using enzymes, thus the wheat bran oligosaccharides including five-carbon oligosaccharides and six-carbon oligosaccharides can be prepared, the utilization rate of a variety of components in the wheat bran can be increased, the generation of wastes can be reduced, and the pollution to environment can be reduced.

Ultrafine-grinding assisted enzymatic-hydrolysis based preparation method of wheat bran oligosaccharides

The invention discloses immunopeptide freeze-dried powder and a preparation method thereof, belongs to the technical fields of deep-processing of agricultural products and comprehensive utilization of by-products thereof, and relates to immunopeptide freeze-dried powder and a preparation method thereof. With any one of corn gluten meal, wheat protein powder, rice protein powder, soyabean protein powder, mung bean protein powder and poultry and egg white powder as a raw material, a process route comprising superfine grinding of designed raw material, blending and homogenizing of a protein solution, controlled enzymatic hydrolysis under an environment of a high-voltage pulsed electric field, low-temperature enzyme deactivation, low-temperature centrifugation, low-temperature membrane separation and vacuum freeze drying is realized, wherein the moisture content of the raw material is 1.0-5.0% and the protein content of the raw material is 40-70%; the immunopeptide freeze-dried powder of which the moisture content is 1.2-1.8%, the protein content is 72-86% and the molecular weight is 10-30 kDa is obtained. Compared with a control group, an immune activity evaluation experiment proves that the multiplication capacity of splenic lymphocyte of mice of an experimented group is significantly improved.

Immunopeptide freeze-dried powder and preparation method thereof

A reduced sugar syrup having an advantageously low viscosity is prepared by hydrolysis of starch or starchy material using a particular type of alpha amylase enzyme which yields a saccharide distribution having a low DP1-2 and low DP11 + content. The DP4 content of the syrup may be favorably increased by using a maltotetragenic alpha amylase enzyme in combination with the aforementioned alpha amylase enzyme. The syrup is useful in the production of food, beverage, animal feed, animal health and nutrition, pharmaceutical, and cosmetic compositions and may be combined with a high intensity sweetener to provide a composition capable of being substituted for conventional corn syrups.

Reduced sugar syrups and methods of making reduced sugar syrups

The invention discloses a preparation method of resistant dextrin. The method includes the following steps that acid-treated starch is used as a raw material, the raw material is placed in a hopper onthe lower portion of a boiling dryer, after being purified and heated, dry gas passes through a gas distribution plate on the bottom of the hopper and enters a main tower of the boiling dryer, the raw material is in a fluidized and boiled state under stirring and negative-pressure effects, and the raw material and high-temperature air flow formed by the dry gas are subjected to an acid-heat reaction in the mass transfer and heat transfer process; the raw material carried away by the high-temperature air flow is captured by a cloth bag arranged on the upper portion of the main towel under theeffect of a draught fan, through bag shaking under the effect of pulse of a dust cleaning mechanism arranged on the cloth bag, the raw material returns to the main tower for acid-heat reaction again,after the acid heat reaction, a pyrodextrin crude product is obtained, and the resistant dextrin product is obtained after the refining process. The preparation method can be continually and stably operated, the thermal efficiency is high, starch can make contact with the high-temperature air flow to the utmost extent, the resistant dextrin is heated uniformly and not easily carbonized, the reaction time is short, the process is simple and the cost is low.

Preparation method of resistant dextrin

The invention provides a processing method of resistant dextrin. According to the processing method, starch is taken as a raw material, resistant dextrin is obtained after acid treatment, sugar liquid spraying, granulation and drying, charring, enzyme hydrolysis, color and salt removal with a chromatographic column, sugar removal refinement and drying sequentially, and a sugar liquid obtained in the sugar removal refinement step is condensed to be used as a raw sugar liquid material for sugar liquid spraying. The sugar liquid separated in the sugar removal refinement step is condensed to be used as the raw sugar liquid material to be mixed with the starch, the yield of the product is increased by 10%-30%, and the production cost is reduced; the granulation process is added, the uniformity degree of heat treatment is effectively increased, and the charring effect and uniformity are increased.

Processing method of resistant dextrin

Method for preparing high-purity isomaltooligosacharide from wheat starch

The invention relates to a method of taking bran as raw materials to prepare high-purity low-poly araboxylan and ferulic acid and belongs to the field of biomass comprehensive utilization. According to the invention, the bran of wheat and corns are subjected to efficient pre-processing and an ultrasonic assisted composite enzymolysis is adopted so as to obtain a mixed liquid of the araboxylan and the ferulic acid, and then the mixed liquid flows through absorbent carbon to be subjected to adsorption and separation; the araboxylan solution is subjected to bleaching through the combination of absorbent carbon particles and bleaching resin, and subjected to chromatographic fractionation through a sequential simulated moving bed, so as to obtain the low-poly araboxylan product with oligosaccharide purity higher than 95 percent; and the ferulic acid is subjected to ethanol leaching to obtain the high-purity ferulic acid product. According to the invention, the bran, by-products of grain processing, are taken as raw materials for deep processing and comprehensive utilization, thereby obtaining two products which have high added value, are high in purity and good in functionality; and accordingly, the food raw material utilization rate is effectively improved, and the added value of the deep processing industry of the food is increased.

Method of taking bran as raw materials to prepare high-purity low-poly araboxylan and ferulic acid

The invention discloses a high-yield method for co-producing the resistant dextrin, the beta-cyclodextrin and the F42 HFCS. According to the process, corn starch serves as a raw material, digestible dextrin is converted into the beta-cyclodextrin through burning, liquefaction and addition of cyclodextrin glucoxyltransferase, and composite insoluble substances are obtained through toluene composition and filtered, toluene is recycled and the beta-cyclodextrin is obtained; the surplus solution is subjected to composite saccharifying enzyme saccharification, chromatographic separation and refining, and resistant dextrin and corn syrup are obtained; and the corn syrup is subjected to isomerization and fining, and the F42 HFCS is obtained. According to the method, the high-purity resistant dextrin can be produced, the application field of the resistant dextrin is expanded, the surplus digestible mother solution is effectively used to produce the beta-cyclodextrin and the F42 HFCS, the production cost is greatly reduced, and the material usage rate and the yield are improved apparently.

Wang Naiqiang

Papers

Method for preparing high-purity isomaltooligosacharide from wheat starch

Method of taking bran as raw materials to prepare high-purity low-poly araboxylan and ferulic acid

High-yield method for co-producing resistant dextrin, beta-cyclodextrin and F42 HFCS (high fructose corn syrup)

Production method of isomaltooligosacharide and animal feed additive

Method for co-producing crystalline fructose and fructose corn syrup by taking wheat as raw material, and preparation method of wheat starch