Strobilurins are one of the most important classes of agricultural fungicide. Their invention was inspired by a group of fungicidally active natural products. The outstanding benefits they deliver are currently being utilised in a wide range of crops throughout the world. First launched in 1996, the strobilurins now include the world's biggest selling fungicide, azoxystrobin. By 2002 there will be six strobilurin active ingredients commercially available for agricultural use. This review describes in detail the properties of these active ingredients--their synthesis, biochemical mode of action, biokinetics, fungicidal activity, yield and quality benefits, resistance risk and human and environmental safety. It also describes the clear technical differences that exist between these active ingredients, particularly in the areas of fungicidal activity and biokinetics.

The strobilurin fungicides.

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Myths and Dogmas of Biocontrol Changes in Perceptions Derived from Research on Trichoderma harzinum T-22.

Wine grapes present a unique biogeography model, wherein microbial biodiversity patterns across viticultural zones not only answer questions of dispersal and community maintenance, they are also an inherent component of the quality, consumer acceptance, and economic appreciation of a culturally important food product. On their journey from the vineyard to the wine bottle, grapes are transformed to wine through microbial activity, with indisputable consequences for wine quality parameters. Wine grapes harbor a wide range of microbes originating from the surrounding environment, many of which are recognized for their role in grapevine health and wine quality. However, determinants of regional wine characteristics have not been identified, but are frequently assumed to stem from viticultural or geological factors alone. This study used a high-throughput, short-amplicon sequencing approach to demonstrate that regional, site-specific, and grape-variety factors shape the fungal and bacterial consortia inhabiting wine-grape surfaces. Furthermore, these microbial assemblages are correlated to specific climatic features, suggesting a link between vineyard environmental conditions and microbial inhabitation patterns. Taken together, these factors shape the unique microbial inputs to regional wine fermentations, posing the existence of nonrandom “microbial terroir” as a determining factor in regional variation among wine grapes.

https://www.pnas.org/content/pnas/111/1/E139.full.pdf

Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate

Oomycetes form a deep lineage of eukaryotic organisms that includes a large number of plant pathogens which threaten natural and managed ecosystems. We undertook a survey to query the community for their ranking of plant-pathogenic oomycete species based on scientific and economic importance. In total, we received 263 votes from 62 scientists in 15 countries for a total of 33 species. The Top 10 species and their ranking are: (1) Phytophthora infestans; (2, tied) Hyaloperonospora arabidopsidis; (2, tied) Phytophthora ramorum; (4) Phytophthora sojae; (5) Phytophthora capsici; (6) Plasmopara viticola; (7) Phytophthora cinnamomi; (8, tied) Phytophthora parasitica; (8, tied) Pythium ultimum; and (10) Albugo candida. This article provides an introduction to these 10 taxa and a snapshot of current research. We hope that the list will serve as a benchmark for future trends in oomycete research.

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The Top 10 oomycete pathogens in molecular plant pathology

The microbial ecology of wine grape berries.

SUMMARY Few plant pathogens have had a more profound effect on the evolution of disease management than Erysiphe necator, which causes grapevine powdery mildew. When the pathogen first spread from North America to England in 1845, and onwards to France in 1847, ‘germ theory’ was neither understood among the general populace nor even generally accepted within the scientific community. Louis Pasteur had only recently reported the microbial nature of fermentation, and it would be another 30 years before Robert Koch would publish his proofs of the microbial nature of certain animal diseases. However, within 6 years after the arrival of the pathogen, nearly 6 million grape growers in France were routinely applying sulphur to suppress powdery mildew on nearly 2.5 million hectares of vineyards (Campbell, 2006).The pathogen has remained a focus for disease management efforts ever since. Because of the worldwide importance of the crop and its susceptibility to the disease, and because conventional management with modern, organic fungicides has been compromised on several occasions since 1980 by the evolution of fungicide resistance, there has also been a renewed effort worldwide to explore the pathogen’s biology and ecology, its genetics and molecular interactions with host plants, and to refine current and suggest new management strategies. These latter aspects are the subject of our review. Taxonomy: The most widely accepted classification follows. Family Erysiphaceae, Erysiphe necator Schw. [syn. Uncinula necator (Schw.) Burr., E. tuckeri Berk., U. americana Howe and U. spiralis Berk. & Curt; anamorph Oidium tuckeri Berk.]. Erysiphe necator var. ampelopsidis was found on Parthenocissus spp. in North America according to Braun (1987), although later studies revealed isolates whose host range spanned genera, making the application of this taxon somewhat imprecise (Gadoury and Pearson, 1991). The classification of the genera before 1980 was based on features of the mature ascocarp: (i) numbers of asci; and (ii) morphology of the appendages, in particular the appendage tips. The foregoing has been supplanted by phylogeny inferred from the internal transcribed spacer (ITS) of ribosomal DNA

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Grapevine powdery mildew (Erysiphe necator): a fascinating system for the study of the biology, ecology and epidemiology of an obligate biotroph.

Potential for biological control of Phytophthora root and crown rots of apple by Trichoderma and Gliocladium spp.

Berries of Vitis vinifera are reported to be susceptible to infection by Uncinula necator until soluble solids levels (brix) reach 8%, and established colonies are reported to sporulate until brix reach 15%. However, our analysis of disease progress on fruit of selected V. vinifera cultivars indicated that severity became asymptotic several weeks earlier in fruit development. When mildew-free fruit clusters of V. vinifera ‘Chardonnay’, ‘Riesling’, ‘Gewurztraminer’, and ‘Pinot Noir’ were inoculated at stages ranging from prebloom to 6 weeks postbloom, only fruit inoculated within 2 weeks of bloom developed severe powdery mildew. Substantial ontogenic resistance to infection was expressed in fruit nearly 6 weeks before fruit brix reached 8% and over 2 months before they reached 15%. Rachises of ‘Chardonnay’ and ‘Riesling’ fruit clusters developed severe powdery mildew when inoculated at bloom, and disease increased steadily over the next 60 days. The rachis of fruit clusters inoculated 31 days afte...

https://apsjournals.apsnet.org/doi/pdfplus/10.1094/PHYTO.2003.93.5.547

Ontogenic resistance to powdery mildew in grape berries.

The physical modes of action of azoxystrobin, mancozeb, and metalaxyl were evaluated on grapevine seedlings using Plasmopara viticola as a model pathogen. The protectant, postinfection, postsymptom, translaminar, and vapor activities of azoxystrobin were evaluated at a rate of 250 μg/ml. Azoxystrobin provided 100% disease control when applied 1 to 5 days before inoculation. Postinfection applications of azoxystrobin had little effect on the incidence of disease, but colony area and sporulation from the resultant lesions was reduced by 47 and 96%, respectively, relative to the check treatment when applied up to 5 days after inoculation. Postsymptom applications (6 days after inoculation) of azoxystrobin resulted in an 85% mean reduction of resporulation from diseased tissue relative to the check when seedlings were evaluated 1 to 14 days after treatment. Translaminar activity was greatest when the upper surface of the leaf was treated 7 days before inoculation of the lower leaf surface (94% disease control). In contrast, control was <50% when leaves were similarly inoculated 1 and 3 days after treatment. Vapor activity was not pronounced, providing maximum reductions of 5, 11, and 37%, with regard to incidence, colony area, and sporulation, relative to the check when seedlings were treated 1 to 7 days before inoculating adjacent, untreated leaves. Comparatively, mancozeb (1,790 μg/ml) provided complete control of the disease when applied 1 to 5 days before inoculation, but showed little postinfection activity in reducing disease incidence, although it exhibited moderate to high antisporulant activity when applied in postinfection and postsymptom modes (mean reductions of 38 and 89%, respectively, compared with the check treatments). Metalaxyl (260 μg/ml) also provided complete control of the disease when used in protectant mode, and also when applied 1 day after inoculation. Applications at 3 to 5 days after inoculation provided substantial reductions in disease severity and sporulation (mean reductions of 46 and 94%, respectively, compared with the check treatments), and postsymptom applications resulted in a mean 84% reduction in resporulation. Collectively, the results of this study illustrate the unique physical modes of action for azoxystrobin in comparison to that of two traditional protectant and systemic fungicides, and provide information on how azoxystrobin and other strobilurin fungicides with similar physical modes of action should be best used in disease management programs.

https://apsjournals.apsnet.org/doi/pdfplus/10.1094/PDIS.2001.85.6.649

Comparative Physical Modes of Action of Azoxystrobin, Mancozeb, and Metalaxyl Against Plasmopara viticola (Grapevine Downy Mildew)

Two hundred fifty-six single-conidial chain isolates of Uncinula necator were assayed for their sensitivity to azoxystrobin and myclobutanil. These isolates were collected from two sites in New York in 1999: an "organic" vineyard where no synthetic fungicides have been used (baseline population) and a commercial vineyard having a history of compromised powdery mildew control with myclobutanil (demethylation inhibitor [DMI]-resistant population). Mean coefficients of variance for a leaf disk assay used to test fungicide sensitivities were 31% for azoxystrobin and 41% for myclobutanil. Baseline ED50 values ranged from 0.0037 to 0.028 μg/ml (mean 0.0097μg/ml) for azoxystrobin and from 0.0049 to 0.69 μg/ml (mean 0.075 μg/ml) for myclobutanil. A shift in the mean ED50 value for azoxystrobin to 0.018 μg/ml was observed in the DMI-resistant population; with the strongest shift observed for isolates collected from vines treated exclusively with myclobutanil (0.024 μg/ml). For the 256 tested isolates, there was a moderate, but statistically significant, correlation between azoxystrobin and myclobutanil sensitivities (R2 = 0.36, P < 0.001). Tests with three other strobilurin fungicides (kresoxim-methyl, pyraclostrobin, and trifloxystrobin) indicate clear differences in the intrinsic activity of these compounds against U. necator, and the applicability of the methods developed with azoxystrobin for assays with pyraclostrobin and trifloxystrobin. Isolates from the high and low ends of the azoxystrobin sensitivity distribution (15× difference in mean ED50 values) were equally controlled in planta by protectant or postinfection treatment with azoxystrobin at 250 μg a.i./ml, but postinfection application at lower rates (2.5 and 25 μg a.i./ml) resulted in a 41 and 44% decrease, respectively, in the control of the low-sensitivity isolates versus high-sensitivity isolates. The results of this study document the baseline sensitivity distribution of U. necator to azoxystrobin, provide evidence of partial cross-sensitivity between azoxystrobin and myclobutanil, and illustrate the potential selection for individuals with reduced sensitivity (quantitative range) to azoxystrobin by postinfection application and reduced rates of this fungicide.

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Wayne F. Wilcox

Papers

Grapevine powdery mildew (Erysiphe necator): a fascinating system for the study of the biology, ecology and epidemiology of an obligate biotroph.

Potential for biological control of Phytophthora root and crown rots of apple by Trichoderma and Gliocladium spp.

Ontogenic resistance to powdery mildew in grape berries.

Comparative Physical Modes of Action of Azoxystrobin, Mancozeb, and Metalaxyl Against Plasmopara viticola (Grapevine Downy Mildew)

Sensitivity to Azoxystrobin Among Isolates of Uncinula necator: Baseline Distribution and Relationship to Myclobutanil Sensitivity.