The invention relates to a method for extracting piperlonguminine from long pepper. The method comprises the following steps of: crushing long pepper medicinal material serving as a raw material; adding alcohol to soak for 1-2 hours; adding into an extraction kettle; collecting piperlonguminine extract by using supercritical CO2 extraction; dissolving with acid water; filtering off insoluble; adding alkali into the filtrate to adjust the pH to be 8-10; adding chloroform to extract; recovering the chloroform from the extract; performing separation and purification by using high-speed counter-current chromatography; concentrating effluent; and performing low-temperature drying to obtain a product. The method has the advantages of high extraction efficiency, large preparation capacity, less sample loss and high product purity.

Method for extracting piperlonguminine from long pepper

Method for simultaneous determination of piperine content and piperlonguminine content in long piper extract through HPLC (high performance liquid chromatography)

The invention relates to a Mongolian medicine long pepper medicine efficacy material basis analysis method. The method comprises the following steps that (i) Mongolian medicine long pepper extracts are prepared, commercially available Mongolian medicine of dried long pepper fruits are purchased and are subjected to ultramicro pulverization, an ethanol solution with the ethanol mass percent being 65 to 100 wt percent is added, ultrasonic extraction is carried out at 40 to 45 DEG C, the time is 4 to 6 hours, the filtering is carried out, and an extracting solution is obtained; (ii) the medicine efficacy material basis of the Mongolian medicine long pepper extracts is subjected to qualitative analysis; an efficient liquid chromatography is used for carrying out qualitative analysis on medicine efficacy material basis ingredients of the Mongolian medicine long pepper extracts, the prepared Mongolian medicine long pepper extracts are dissolved in flowing phases, the separation is carried out through the efficient liquid chromatography, the obtained chromatogram is compared with the chromatogram of the long pepper medicine effect ingredient standard product, and the ingredients of the Mongolian medicine long pepper extracts are analyzed. The method has the advantages that the operation is simple, various medicine effect ingredients of long pepper can be extracted, in addition, the extracting rate of the medicine effect active ingredients is improved, and the method is suitable for industrial production.

Mongolian medicine long pepper medicine efficacy material basis analysis method

The invention relates to a Mongolian medicine piper longum extract used for reducing blood fat. The piper longum extract is prepared through following steps: (1) buying market mongolian medicine, dry piper longum fruits, and ultrafine-pulverizing the dry piper longum fruits, adding an ethanol solution being 65-100 wt% in mass percentage, performing ultrasonic extraction at 40-45 DEG C for 4-6 h, and performing filtration to obtain an extract liquid; (2) performing silica gel chromatographic column adsorption to the extract liquid, performing elution with water by three times of the volume of the column, performing elution with methanol-chloroform as an elution liquid and collecting the eluant; (3) performing pressure-reduced concentration to the eluant to obtain a dry extract; (4) dissolving the dry extract with an acetone solution being 75-80 wt% in mass percentage, passing the solution through a macroporous adsorption resin column, performing elution with an acetone solution being 10-20 wt% in mass percentage by three times of the volume of the column and rejecting an eluant, and performing elution with an acetone solution being 75-80 wt% in mass percentage and collecting an eluant; and (5) performing pressure-reduced concentration to the eluant and drying a product to obtain the mongolian medicine piper longum extract.

Mongolian medicine piper longum extract used for reducing blood fat

The invention belongs to the technical field of medicine preparation, relates to an extracting and separating method of medicinal plant chemical component, and particularly relates to a chemical component separating part of methylene dichloride extracting part of piper longum composition and its application. The method includes steps of extracting piper longum composition through a carbon dioxidesupercutical fluid method and removing oil; extracting drug slag by 70% of ethanol, concentrating and extracting by methylene dichloride; recycling the methylene dichloride and drying to obtain a methylene dichloride extracting part; preparing liquid chromatography, reverse-phased ODS column chromatography, LH-20 gel column chromatography and thin-layer chromatography by a normal phase silica gelcolumn chromatography under high pressure to systematically separate the chemical component of the methylene dichloride extracting part, so as to obtain 10 kinds of compounds. The pharmacodynamic test result indicates that the 10 kinds of compounds separated from the methylene dichloride extracting part of piper longum composition has protection activity to neutral cell, wherein the activity of dibutyl phthalate in ten compounds is the best. The method is significant to research and development, and quality control of drugs for treating cerebral apoplexy.

Wu Xingrong

Papers

Method for extracting PiPerlonguminine and drug combination comprising same