Tobamovirus-resistant tobacco generated by RNA interference directed against host genes.

Several field isolates of Pepper mild mottle virus (PMMoV) were obtained from Capsicum annuum (L

 3
 
/ + ) that exhibited systemic mosaic or systemic necrosis The deduced amino acid sequences of the coat protein (CP) of these isolates differed from those of previously reported PMMoV strains that are able to overcome L

 3
 gene-mediated resistance CP mutants having these amino acid changes were constructed using cDNA clones pTPWl and pTPC4350 These clones produce in vitro transcripts TPW1, which induces a resistance response resulting in local infection, and TPC4350, which causes no response on inoculated leaves resulting in systemic infection in the Capsicum plants carrying the L

 3
 gene (L

 3
 
/L

 3
 
) These CP mutants were tested for infection and symptomatology in Capsicum spp plants (L

 3
 
/L

 3 and L

 3
 
/ + ) An A/S (from serine to alanine) change at position 81 in the CP of TPW1 conferred a resistance-breaking activity in the L

 3
 
/L

 3
 plants, whereas an S/A change at position 7 in the CP of either TPW1 or TC4350 conferred resistance-inducing activities These results suggest that amino acid changes of A/S at position 81 and S/A at position 7 in CP had inverse effects on the L

 3
 gene-mediated resistance These and other amino acid changes in PMMoV CP are also discussed in relation to the dosage effects of the L

 3
 gene on virus resistance

Amino Acid Changes in Pepper mild mottle virus Coat Protein That Affect L3 Gene-mediated Resistance in Pepper'

The complete nucleotide sequence of an attenuated Pepper mild mottle virus (PMMoV C-1421) RNA genome has been determined. There were two differences from the type isolate in Japan (PMMoV-J). The mutations were located in the middle of the 126-kDa protein (126 K) gene; one mutation influenced amino acid substitution at 649th Val to Ala (V649A), and the other was silent. The analyses using the reverse genetic system of PMMoV-J revealed that symptom attenuation on pepper related to V649A. Accumulations of 126 K and coat protein (CP) in V649A mutant-infected pepper were lower than those of PMMoV-J in immunoblotting. These results suggest that V649A substitution in 126 K affects the accumulation of 126 K leading to a limitation of CP accumulation.

A single amino acid substitution in 126-kDa protein of Pepper mild mottle virus associates with symptom attenuation in pepper; the complete nucleotide sequence of an attenuated strain, C-1421.

Pepper mild mottle virus (PMMoV) infects pepper plants, causing mosaic symptoms on the upper developing leaves. We investigated the relationship between a virus pathogenicity determinant d...

https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-97-4-0412

Pathogenicity of Pepper mild mottle virus Is Controlled by the RNA Silencing Suppression Activity of Its Replication Protein but Not the Viral Accumulation.

Severe systemic viral symptoms were observed on the leaves of infected pepper ( Capsicum annuum L.) plants cultivated in Antalya located in the Mediterranean coast of Turkey, in 2008. The symptoms on the diseased pepper plants included, mosaic, mottle, chlorosis coupled with stunting, chlorotic spots, distortion of the leaves and fruits. Using Pepper mild mottle tobamovirus (PMMoV) specific polyclonal antisera, PMMoV was detected in symptomatic pepper plants by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Disease occurrence (the percentage of plants infected) of up to 30% was estimated in the region outbreaks. It was observed that, symptomatic pepper samples collected from Antalya province were infected with PMMoV (47.14%) according to the results of the study. Key words : Virus, pepper, outbreak, double antibody sandwich-enzyme linked immunosorbent assay (DASELISA), Pepper mild mottle tobamovirus (PMMoV), disease occurrence.

/pdf/occurrence-of-pepper-mild-mottle-virus-in-greenhouse-grown-2laqezq31f.pdf

雄一郎渡辺

Papers

Nucleotide Sequence of the Japanese Isolate of Pepper Mild Mottle Tobamovirus (TMV-P) RNA.

雄一郎 渡辺

Papers

Nucleotide Sequence of the Japanese Isolate of Pepper Mild Mottle Tobamovirus (TMV-P) RNA.

雄一郎渡辺