Effect of various selenium doses on chromium(IV)-induced nephrotoxicity in a male chicken model

Cr(VI) is a widely used chemical. Excessive Cr(VI) exposure not only causes inflammatory damage but also induces mitophagy. This study aimed to demonstrate the effect of Cr(VI) on inflammatory injury and mitophagy in chicken liver. A total of 120 Hyland Brown cockerels (1 day old) were randomly divided into four groups and orally treated with different Cr(VI) doses (10% median lethal dose, 6% median lethal dose, 2% median lethal dose, and 0% median lethal dose) daily for 45 days to explore the underlying mechanism. Results showed that excessive Cr(VI) increased tumor necrosis factor-α, interleukin-6, and heat shock protein but decreased interferon-γ expression and adenosine triphosphate content in chicken liver. Cr(VI) significantly increased reactive oxygen species production, induced mitochondrial membrane potential collapse, and promoted autophagosome formation. Cr(VI) treatment also caused an increase in LC3-II, stimulated Parkin translocation, and inhibited the expression of p62/SQSTM1 and translocase of outer mitochondrial membrane 20. Therefore, excessive Cr(VI) caused inflammatory damage and mitophagy in chicken liver.

Inflammatory injury and mitophagy induced by Cr(VI) in chicken liver.

Platycodon grandifloras polysaccharides inhibit mitophagy injury induced by Cr (VI) in DF-1 cells

Article history Received: 19 Apr, 2015 Revised: 22 May, 2015 Accepted: 24 May, 2015 Abstract This study evaluated hepatotoxicity induced by potassium dichromate in chickens via drinking water. Seventy-two Hyland Brown male chickens were randomly divided into 4 groups (18 chickens each group): Control group, low-dose (2%LD50) Cr(VI) group, middle-dose (6%LD50) Cr(VI) group, high-dose (10%LD50) Cr(VI) group. Potassium dichromate was given orally via drinking water to the chickens for 42 day. The control group was treated with the same volume of distilled water. Blood biochemical indexes (ALT, AST, GGT), antioxidant indicators (SOD, GSH and MDA) in liver homogenates and pathologic changes in tissues were detected at 14, 28 and 42 day respectively. The levels of serum biochemical indexes (ALT, AST, GGT) increased significantly (P<0.05) in Cr(VI)-treated groups. The activities of SOD and GSH decreased significantly (P<0.05) in the Cr(VI)-treated groups while the content of MDA increased significantly (P<0.05) in the Cr(VI)-treated groups compared to control with the increasing dose and time. The cells of the liver treated with Cr(VI) were severely dilated and the liver appeared severe inflamed and necrotic. The results indicated that Cr(VI) can lead to hepatotoxicity damage in chicken with time and dosedependent relationship.

Xu Wang

Papers

Hepatotoxicity induced by potassium dichromate in chickens.