The invention provides a verbena bonariensis cultivation method. The verbena bonariensis cultivation method comprises a pre-treatment step, a low-temperature sleeping treatment stage, a seed germination stage, a strong seedling transplanting stage, and a nutritional solution culture stage. The method is simple and is easy to operate; seedling development time is relatively short and the specification consistency is high; and the dosage of compounds is greatly reduced, the pollution generated by a nutritional solution is reduced, and the tissue culture effect is good.

Verbena bonariensis cultivation method

The invention discloses a rapid propagation method for mutation breeding of herba verbena officinalis. The method comprises steps of disinfection of seeds, mutation of the seeds, induction of callus tissue, differentiation of callus tissue, rooting induction and the like. According to the method, the soluble protein content of seedlings can be increased through irradiation of ion beams, the protective enzyme system activity is improved, the seed stress resistance is improved, the genetic stability is good, and the regeneration rate of seedlings is high.

Yang Chengdong

Papers

Rapid propagation method for mutation breeding of herba verbena officinalis