Showing papers by "Yayoi Nishiyama published in 1997"
TL;DR: The cytological alteration induced by exposure to a higher concentration (40 μg/ml) of the drug was characterized by disruption of the intracytoplasmic organelles, confirming the strong antifungal activity of OMZ against fungal cells.
Abstract: The antifungal effects of an imidazole-antimyeotic omoconazole nitrate (OMZ) on the morphology and ultrastructure of Candida albicans yeast cells were studied using scanning and transmission electron microscopy The treatment of growing Candida cultures with fungistatic doses (04 to 4 μg/ml) of OMZ produced the formation of a chain or cluster of cells Thickening of the cell wall and accumulation of electron-dense vesicles in the wall were clearly observed Development of Golgi-like complex membranous structures in the cytoplasm was the most prominent finding The cytological alteration induced by exposure to a higher concentration (40 μg/ml) of the drug was characterized by disruption of the intracytoplasmic organelles Our results confirm the strong antifungal activity of OMZ against fungal cells
12 citations
Patent•
06 Jun 1997
TL;DR: In this article, a fungus such as Aspergillus fumigatus is exposed to the agent at 1-400 mg/l in a sealed space by heat evaporation and so on, preferably for 2-3 days to prevent spore-formation or for 10-30 min to suppress growth of its mycelium and regrowth of it.
Abstract: PROBLEM TO BE SOLVED: To obtain the subject agent having low toxicity against warm-blooded animals, inhibiting spore-forming activity of sporogenic fungi, terminating growth of its mycelium and re-growth of it, and hence applicable to sickrooms which house easily infections patients, by including essential oil land so on. SOLUTION: This agent is obtained by including an essential oil such as perilla oil, pieces of a plant (e.g. leaf of green perilla) containing an essential oil, or main components of an essential oil such as carvacrol and timor. A fungus such as Aspergillus fumigatus is exposed to the agent at 1-400 mg/l in a sealed space by heat evaporation and so on, preferably for 2-3 days to prevent spore-formation or for 10-30 min to suppress growth of its mycelium and re-growth of it. Other applicable places include ones to store crop/food and bath room. COPYRIGHT: (C)1998,JPO
8 citations
TL;DR: Electrophoretic analysis showed that protease caused a loss of cell wall‐lytic activity of the cell, which possibly led to the formation of multicells through cessation of cross wall separation.
Abstract: Multicellular cells were efficiently induced in Staphylococcus haemolyticus by the addition of protease to exponentially growing cultures at 30 C Electron microscopy revealed the formation of tetrad-shaped multicells that were septated but not separated from each other Incubation of the multicells with extract from the cells grown without protease resulted in a fourfold increase in the number of colony-forming units as compared with the untreated control An electrophoretic analysis showed that protease caused a loss of cell wall-lytic activity of the cell, which possibly led to the formation of multicells through cessation of cross wall separation
5 citations