다중혈관 관상동맥 환자에서 y-문합을 이용하여 양쪽 내흉동맥만을 사용한 우회술의 조기 성적

Probability Distributions.- Linear Models for Regression.- Linear Models for Classification.- Neural Networks.- Kernel Methods.- Sparse Kernel Machines.- Graphical Models.- Mixture Models and EM.- Approximate Inference.- Sampling Methods.- Continuous Latent Variables.- Sequential Data.- Combining Models.

Pattern Recognition and Machine Learning

Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) by expression of defined embryonic factors. However, little is known of the molecular mechanisms underlying the reprogramming process. Here we explore somatic cell reprogramming by exploiting a secondary mouse embryonic fibroblast model that forms iPSCs with high efficiency upon inducible expression of Oct4, Klf4, c-Myc, and Sox2. Temporal analysis of gene expression revealed that reprogramming is a multistep process that is characterized by initiation, maturation, and stabilization phases. Functional analysis by systematic RNAi screening further uncovered a key role for BMP signaling and the induction of mesenchymal-to-epithelial transition (MET) during the initiation phase. We show that this is linked to BMP-dependent induction of miR-205 and the miR-200 family of microRNAs that are key regulators of MET. These studies thus define a multistep mechanism that incorporates a BMP-miRNA-MET axis during somatic cell reprogramming. PAPERCLIP:

Functional genomics reveals a bmp driven mesenchymal-to-epithelial transition in the initiation of somatic cell reprogramming (oral presentation)

Newly endocytosed integral cell surface proteins are typically either directed for degradation or subjected to recycling back to the plasma membrane The sorting of integral cell surface proteins, including signalling receptors, nutrient transporters, ion channels, adhesion molecules and polarity markers, within the endolysosomal network for recycling is increasingly recognized as an essential feature in regulating the complexities of physiology at the cell, tissue and organism levels Historically, endocytic recycling has been regarded as a relatively passive process, where the majority of internalized integral proteins are recycled via a nonspecific sequence-independent 'bulk membrane flow' pathway Recent work has increasingly challenged this view The discovery of sequence-specific sorting motifs and the identification of cargo adaptors and associated coat complexes have begun to uncover the highly orchestrated nature of endosomal cargo recycling, thereby providing new insight into the function and (patho)physiology of this process

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To degrade or not to degrade: mechanisms and significance of endocytic recycling.

Clathrin adaptor epsinR is required for retrograde sorting on early endosomal membranes

Retromer is a peripheral membrane protein complex that coordinates multiple vesicular trafficking events within the endolysosomal system. Here, we demonstrate that retromer is required for the maintenance of normal lysosomal morphology and function. The knockout of retromer subunit Vps35 causes an ultrastructural alteration in lysosomal structure and aberrant lysosome function, leading to impaired autophagy. At the whole-cell level, knockout of retromer Vps35 subunit reduces lysosomal proteolytic capacity as a consequence of the improper processing of lysosomal hydrolases, which is dependent on the trafficking of the cation-independent mannose 6-phosphate receptor (CI-M6PR). Incorporation of CI-M6PR into endosome transport carriers via a retromer-dependent process is restricted to those tethered by GCC88 but not golgin-97 or golgin-245. Finally, we show that this retromer-dependent retrograde cargo trafficking pathway requires SNX3, but not other retromer-associated cargo binding proteins, such as SNX27 or SNX-BAR proteins. Therefore, retromer does contribute to the retrograde trafficking of CI-M6PR required for maturation of lysosomal hydrolases and lysosomal function.

https://rupress.org/jcb/article-pdf/218/2/615/1379711/jcb_201806153.pdf

Retromer has a selective function in cargo sorting via endosome transport carriers.

The wide area monitoring system (WAMS) is considered a pivotal component of future electric power grids. As a pilot WAMS that has been operated for more than a decade, the frequency monitoring network FNET/GridEye makes use of hundreds of global positioning system-synchronized phasor measurement sensors to capture the increasingly complicated grid behaviors across the interconnected power systems. In this paper, the FNET/GridEye system is overviewed and its operation experiences in electric power grid wide area monitoring are presented. Particularly, the implementation of a number of data analytics applications will be discussed in details. FNET/GridEye lays a firm foundation for the later WAMS operation in the electric power industry.

A Distribution Level Wide Area Monitoring System for the Electric Power Grid–FNET/GridEye

This study explores the impact of high-photovoltaic (PV) penetration on the inter-area oscillation modes of large-scale power grids. A series of dynamic models with various PV penetration levels are developed based on a detailed model representing the U.S. Eastern Interconnection (EI). Transient simulations are performed to investigate the change of inter-area oscillation modes with PV penetration. The impact of PV control strategies and parameter settings on inter-area oscillations is studied. This paper finds that as PV increases, the damping of the dominant oscillation mode decreases monotonically. It is also observed that the mode shape varies with the PV control strategy and new oscillation modes may emerge under inappropriate parameter settings in PV plant controls.

Impact of High PV Penetration on the Inter-Area Oscillations in the U.S. Eastern Interconnection

In this paper, we present an update to the models used to calculate the remaining life of transformer paper insulation. A drawback to the current IEEE method is that it does not take into account the availability of water and oxygen on the life expectancy of Kraft paper insulation. We therefore set out to test an algorithm which does take these into account. For our investigation, we loaded three test transformers and ran them to near their end of life. Kinetic equations were applied to model the fall in the degree of polymerization of paper. Our model showed better agreement with the test results than that given from using the IEEE standard. The IEEE standard gives life expectancy for Kraft paper being aged in minimal oxygen under dry conditions, which is not necessarily representative of an old transformer. Wet paper and using high levels of oxygen can age nearly 40 times faster. The IEEE method needs to take the synergistic effect of water and oxygen on increasing the rate of paper aging into account.

An Updated Model to Determine the Life Remaining of Transformer Insulation

The distribution-level electric network frequency (ENF) extracted from an electric power signal is a promising forensic tool for multimedia recording authentication. Local characteristics in ENF signals recorded in different locations act as environmental signatures, which can be potentially used as a fingerprint for location identification. In this paper, a reference database is established for distribution-level ENF using FNET/GridEye system. An ENF identification method that combines a wavelet-based signature extraction and feedforward artificial neural network-based machine learning is presented to identify the location of unsourced ENF signals without relying on the availability of concurrent signals. Experiments are performed to validate the effectiveness of the proposed method using ambient frequency measurements at multiple geographic scales. Identification accuracy is presented, and the factors that affect identification performance are discussed.

Yi Cui

Papers

Retromer has a selective function in cargo sorting via endosome transport carriers.

A Distribution Level Wide Area Monitoring System for the Electric Power Grid–FNET/GridEye

Impact of High PV Penetration on the Inter-Area Oscillations in the U.S. Eastern Interconnection

An Updated Model to Determine the Life Remaining of Transformer Insulation

Source Location Identification of Distribution-Level Electric Network Frequency Signals at Multiple Geographic Scales